Bioelectrochemical sensing of promethazine with bamboo-type multiwalled carbon nanotubes dispersed in calf-thymus double stranded DNA
Bioelectrochemical sensing of promethazine with bamboo-type multiwalled carbon nanotubes dispersed in calf-thymus double stranded DNA
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DOI：
10.1016/j.bioelechem.2014.05.002
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发表时间：
2014-10-01
影响因子：
5
通讯作者：
Rivas, Gustavo A.
中科院分区：
文献类型：
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作者：
Primo, Emiliano N.;Belen Oviedo, M.;Rivas, Gustavo A.
报道了竹节状多壁碳纳米管(BCNT)修饰双链小牛胸腺DNA(DsDNA)修饰的玻碳电极(GCE/bCNT-dsDNA)对异丙嗪(PMZ)的定量测定。循环伏安测试表明，PMZ在GCE/bCNT-dsDNA上表现出薄膜受限的氧化还原行为，而在乙醇中分散的bCNT修饰的GCE上表现出不可逆的吸附行为。差示脉冲伏安-介质交换吸附溶出实验证实，PMZ与bCNT-dsDNA之间的相互作用主要是疏水作用。这些差异是由于PMZ嵌入到支撑bCNTs的dsDNA中，从PMZ的紫外可见吸收光谱的红移和DFTB水平的量子动力学计算中可以看出这一点。PMZ在GCE/bCNT-dsDNA上的高效聚集使其在纳摩尔水平上的灵敏定量成为可能(灵敏度：(3.50+/-0.05)×10(8)µA·cm(-2)·m-1，检测下限：23 nm)。该生物传感器成功地用于医药产品中PMZ的测定，具有良好的相关性。(C)2014爱思唯尔B.V.保留所有权利。
We report the quantification of promethazine (PMZ) using glassy carbon electrodes (GCE) modified with bamboo-like multi-walled carbon nanotubes (bCNT) dispersed in double stranded calf-thymus DNA (dsDNA) (GCE/bCNT-dsDNA). Cyclic voltammetry measurements demonstrated that PMZ presents a thin film-confined redox behavior at GCE/bCNT-dsDNA, opposite to the irreversibly-adsorbed behavior obtained at GCE modified with bCNT dispersed in ethanol (GCE/bCNT). Differential pulse voltammetry-adsorptive stripping with medium exchange experiments performed with GCE/bCNT-dsDNA and GCE modified with bCNTs dispersed in single-stranded calf-thymus DNA (ssDNA) confirmed that the interaction between PMZ and bCNT-dsDNA is mainly hydrophobic. These differences are due to the intercalation of PMZ within the dsDNA that supports the bCNTs, as evidenced from the bathochromic displacement of UV-Vis absorption spectra of PMZ and quantum dynamics calculations at DFTB level. The efficient accumulation of PMZ at GCE/bCNT-dsDNA made possible its sensitive quantification at nanomolar levels (sensitivity: (3.50 +/- 0.05) x 10(8) mu A.cm(-2).M-1 and detection limit: 23 nM). The biosensor was successfully used for the determination of PMZ in a pharmaceutical product with excellent correlation. (C) 2014 Elsevier B.V. All rights reserved.