Vertically Ordered Mesoporous Silica Film-Assisted Label-Free and Universal Electrochemiluminescence Aptasensor Platform
Vertically Ordered Mesoporous Silica Film-Assisted Label-Free and Universal Electrochemiluminescence Aptasensor Platform
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垂直有序介孔二氧化硅薄膜辅助无标记通用电化学发光适体传感器平台
DOI：
10.1021/acs.analchem.6b03317
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发表时间：
2016-12-06
影响因子：
7.4
通讯作者：
Mao, Yinfei
中科院分区：
文献类型：
--
作者：
Liu, Jinquan;He, Dinggeng;Mao, Yinfei
本文将核酸适体门控系统与生长在氧化铟锡（ITO）电极上的垂直有序介孔氧化硅薄膜（MSFs）相结合，构建了一个简单、易实现、无标记的电化学发光（ECL）适体传感器平台。在这个适配子传感器平台中，适配子可以通过非共价静电吸引作用有效地吸附在胺化的MSFs表面，然后作为一种理想的门控材料来控制捕获在MSFs孔中的发光试剂Ru（bipy）（3）（2+）的阻断和释放。在靶分子存在的情况下，特异性的适配子-靶分子结合可以触发孔的脱帽，释放出Ru（bipy）（3）（2+），并可检测到电化学发光信号的降低。该设计的可行性和通用性进行了验证，采用三个适体结合溶菌酶，腺苷，和K+作为门材料，和检测限被确定为0.06 nM，0.75 nM，和0.5 μ M，分别。这种ECL适体传感器基于简单的竞争程序，设计简单，要求不高，操作快速。此外，不需要对适体进行其他化学修饰，这表明该ECL适体传感器可以仅通过改变适体序列而应用于许多其他靶标检测。
Herein, a simple, facile, and label-free electrochemiluminescence (ECL) aptasensor platform was constructed by integration of aptamer-gated systems and vertically ordered mesoporous silica films (MSFs) grown in suit of indium tin oxide (ITO) electrode. In this aptasensor platform, aptamer could be effectively adsorbed on the surface of aminated MSFs by noncovalent electrostatic attraction and then employed as an ideal gate material to control the blocking and releasing of luminescence reagents (Ru(bipy)(3)(2+)) entrapped within the pores of MSFs. In the presence of target, the specific aptamer-target binding could trigger the uncapping the pores, releasing the Ru(bipy)(3)(2+) with detectable reduced of ECL signal. The feasibility and universality of this design was validated by employing three aptamers that bind to lysozyme, adenosine, and K+ as gate materials, and the detection limits were determined to be 0.06 nM, 0.75 nM, and 0.5 mu M, respectively. This ECL aptasensor, based on the simple competitive procedure, was simple design, undemanding, and fast in operation. In addition, no other chemical modification of the aptamer was required, suggesting that this ECL aptasensor could be applied to many other target detections just by altering the aptamer sequence.