Cattle encephalon glycoside and ignotin reduced white matter injury and prevented post-hemorrhagic hydrocephalus in a rat model of intracerebral hemorrhage.
Cattle encephalon glycoside and ignotin reduced white matter injury and prevented post-hemorrhagic hydrocephalus in a rat model of intracerebral hemorrhage.
复制标题
牛脑苷和牛脑素可减轻脑出血大鼠模型中的白质损伤并预防出血后脑积水
DOI：
10.1038/srep35923
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发表时间：
2016-10-26
影响因子：
4.6
通讯作者：
Feng H
中科院分区：
文献类型：
--
作者：
Li R;Ma K;Zhao H;Feng Z;Yang Y;Ge H;Zhang X;Tang J;Yin Y;Liu X;Tan L;Feng H
脑出血（ICH）继发脑室内出血（IVH）相关的发病率、死亡率和残疾是一个全球性的负担。迄今为止，除了支持性护理之外，没有有效的治疗脑出血的方法。在这项研究中，我们评估了牛脑糖苷和肌球蛋白（CEGI）注射液对脑出血伴脑室扩张（IVH/ICH）大鼠模型的神经保护作用。采用Sprague-Dawley大鼠尾状核注射IV型胶原酶建立IVH/ICH大鼠模型。实验动物随机接受CEGI、单唾液四己糖神经节苷脂（GM-1）或生理盐水治疗。采用改良Garcia评分法、转角试验、髓鞘碱性蛋白（MBP）和微管相关蛋白2 （MAP-2）免疫荧光染色、透射电镜（TEM）、磁共振成像等方法评价CEGI对IVH/ICH模型大鼠的神经保护作用。CEGI治疗可显著缓解脑出血后神经行为功能障碍，减少侧脑室增大，促进血肿吸收，有效上调MBP/MAP-2表达，改善脑出血后白质纤维损伤。我们的研究结果表明，CEGI对IVH/ICH大鼠模型具有显著的神经保护作用。因此，它可以作为临床治疗IVH/ICH的候选药物。
The morbidity, mortality, and disability associated with intraventricular hemorrhage (IVH) secondary to intracerebral hemorrhage (ICH) represent a global burden. To date, there is no effective therapy for ICH other than supportive care. In this study, we assessed the neuroprotective effects of Cattle encephalon glycoside and ignotin (CEGI) injection in a rat model of ICH with ventricular extension (IVH/ICH). The IVH/ICH rat model was induced via injection of type IV collagenase in the caudate nucleus of Sprague-Dawley rats. The experimental animals were randomized to receive CEGI, monosialotetrahexosyl ganglioside (GM-1), or normal saline. The modified Garcia scale, corner turn test, immunofluorescence staining for myelin basic protein (MBP) and microtubule associated protein 2 (MAP-2), transmission electron microscopy (TEM), and magnetic resonance imaging were employed to evaluate the neuroprotective effect of CEGI in the IVH/ICH rat model. CEGI treatment significantly alleviated the neurobehavioral dysfunction, reduced the lateral ventricular enlargement, promoted hematoma absorption, effectively up-regulated MBP/MAP-2 expression, and ameliorated white matter fiber damage post-ICH induction. Our results demonstrate that CEGI has significant neuroprotective effects in a rat model of IVH/ICH. Therefore, it can be used as a candidate drug for the clinical treatment of IVH/ICH.