Cryonecrosis of normal and tumor‐ bearing rat liver potentiated by inflow occlusion
Cryonecrosis of normal and tumor‐ bearing rat liver potentiated by inflow occlusion
复制标题
流入阻塞加剧正常和荷瘤大鼠肝脏的冷冻坏死
作者：
H. Neel;A. Ketcham;W. Hammond
冷冻手术涉及大鼠肝脏的大部分，在有和没有流入阻断的情况下，一般都能很好地耐受。在10只动物中，有8只(80%)成功地根除了肿瘤，这些动物的肝脏肿瘤种植迅速扩大。肝实质的坏死在组织学上是定位的和可重复性的。靶区或邻近未经处理的肝脏未见出血或胆漏。治疗后SGOT、SGPT一过性升高，直接反映冷冻坏死区的严重程度。冷冻时冰球的大小不能预测冰球的大小，但冷冻的速度和深度以及解冻的持续时间会影响冷冻的程度和组织学冷冻坏死的体积。快速冻结和缓慢融化是最致命的。增加两次和三次冷冻(重复冷冻)会增加冷损伤，而使用热电偶测量局部温度表明，缺血会增加冷损伤。
Cryosurgery involving large portions of rat liver with and without inflow occlusion was generally well tolerated. Tumors were successfully eradicated in 8 of 10 (80%) animals with rapidly enlarging hepatic tumor implants. Necrosis of liver parenchyma was localized and reproducible histologically. No hemorrhage or bile leakage was encountered in the target area or in adjacent untreated liver. SGOT and SGPT values rose transiently after treatment and reflected directly the extent of cryonecrosis. The magnitude of the cold insult and, hence, the volume of histologic cryonecrosis were not predictable by the size of the iceball at the time of freezing, but were affected by the rate and depth of freezing and the duration of thawing. Rapid freezing and slow thawing are maximally lethal. The cold insult was increased by the addition of two and three freezes (repetitive freezing) and was enhanced by ischemia as indicated by regional temperature measurements using thermocouples.