The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/83 (H5N2) influenza A viruses: sequence and antigenic analyses.
The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/83 (H5N2) influenza A viruses: sequence and antigenic analyses.
复制标题
强毒力和无毒力 A/Chicken/Pennsylvania/83 (H5N2) 甲型流感病毒的神经氨酸酶：序列和抗原分析。
DOI：
10.1016/0042-6822(85)90226-0
复制
发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Webster,RG
中科院分区：
文献类型：
--
作者：
Deshpande,KL;Naeve,CW;Webster,RG
为了确定禽流感病毒神经氨酸酶（NA）在毒力进化过程中发生的序列变化，我们研究了强毒和无毒的A/Chick/Penn/83（H5 N2）流感病毒的NA。从这些病毒推导的氨基酸序列的比较表明，通过点突变的积累从无毒进化而来的强毒株（Beanet al.，1985），获得了NA中的四个氨基酸变化：一个在跨膜区段中，一个在柄中，两个在头部中。推导的氨基酸序列与人N2 NA的氨基酸序列的比较表明，在Chick/Penn/83 NA的茎中有20个氨基酸的缺失。来自无毒和毒性Chick/Penn/83病毒的NA的抗原性分析显示它们在抗原性上非常密切相关，但可以用两种单克隆抗体在可能涉及NA头部中至少一个氨基酸变化的位点进行区分。抗原性分析还表明Chick/Penn/83 NA与1965 - 1984年分离的其他N2禽流感病毒的NA密切相关，支持了先前的研究，表明NA在禽N2流感病毒中具有相对抗原稳定性。Chick/Penn/83 NA是第一个被测序的禽流感病毒N2 NA基因。这些NA与1957年人N2 NA抗原性密切相关，并且与原型1957年人N2 NA显示出高度的氨基酸序列同源性。这些数据进一步支持了1957年人类H2 N2病毒至少部分来源于禽类的观点。
To define the sequence changes that occurred in an avian influenza virus neuraminidase (NA) during the evolution of virulence, we have studied the NA of the virulent and avirulent A/Chick/Penn/83 (H5N2) influenza viruses. A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Beanet al., 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.