SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
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DOI：
10.1002/term.1694
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发表时间：
2015-11-01
影响因子：
3.3
通讯作者：
Sykova, Eva
中科院分区：
文献类型：
--
作者：
Kubinova, Sarka;Horak, Daniel;Sykova, Eva
用于脊髓损伤治疗的支架的结构和力学性能必须提供组织整合和有效的轴突再生。以前的工作已经证明了SIKVAV(Ser-Ile-Lys-Val-Ala-Val)改性的高度超微孔聚(甲基丙烯酸羟乙酯)(PHEMA)水凝胶具有细胞黏附和促进生长的特性。本研究的目的是优化这种水凝胶的孔隙率和力学性能，以开发适合脊髓组织修复的支架材料。将3种定向孔径为60微米、孔隙率为57%~68%、等效硬度为3~45kPa超微孔PHEMA水凝胶植入脊髓半横断内，观察其与宿主组织的结合情况以及轴突向支架孔内生长的程度。SIKVAV修饰的PHEMA水凝胶的组织反应最好，孔隙率为68%，弹性系数适中(沿孔方向为27kPa，垂直方向为3.6kPa)。当被植入脊髓横断时，水凝胶促进了组织桥接和轴突向内生长。综上所述，SIKVAV修饰的PHEMA水凝胶用于脊髓损伤修复是一种具有前瞻性的支架结构；然而，为了开发一种有效的脊髓损伤治疗方法，需要多种治疗方法。版权所有(C)2013 John Wiley&Sons，Ltd.
The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well as effective axonal regeneration. Previous work has demonstrated the cell-adhesive and growth-promoting properties of the SIKVAV (Ser-Ile-Lys-Val-Ala-Val)-modified highly superporous poly(2-hydroxethyl methacrylate) (PHEMA) hydrogels. The aim of the current study was to optimize the porosity and mechanical properties of this type of hydrogel in order to develop a suitable scaffold for the repair of spinal cord tissue. Three types of highly superporous PHEMA hydrogels with oriented pores of similar to 60 mu m diameter, porosities of 57-68% and equivalent stiffness characterized by elasticity moduli in the range 3-45 kPa were implanted into a spinal cord hemisection, and their integration into the host tissue, as well as the extent of axonal ingrowth into the scaffold pores, were histologically evaluated. The best tissue response was found with a SIKVAV-modified PHEMA hydrogel with 68% porosity and a moderate modulus of elasticity (27 kPa in the direction along the pores and 3.6 kPa in the perpendicular direction). When implanted into a spinal cord transection, the hydrogel promoted tissue bridging as well as aligned axonal ingrowth. In conclusion, a prospective oriented scaffold architecture of SIKVAV-modified PHEMA hydrogels has been developed for spinal cord injury repair; however, to develop an effective treatment for spinal cord injury, multiple therapeutic approaches are needed. Copyright (C) 2013 John Wiley & Sons, Ltd.