Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes
Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes
复制标题
DOI：
10.1016/j.biomaterials.2006.07.005
复制
发表时间：
2006-11-01
期刊：
影响因子：
14
通讯作者：
Goldstein, Aaron S.
中科院分区：
文献类型：
--
作者：
Bashur, Chris A.;Dahlgren, Linda A.;Goldstein, Aaron S.
工程化的韧带组织是修复撕裂和断裂的有前景的材料，但需要开发生物材料支架，这种支架不仅要能承受生理相关的负荷，还需具备能够引导细胞黏附和细胞外基质沉积的结构。基于微米级地形特征通过接触引导现象诱导细胞定向的证据，我们假定由静电纺丝过程形成的定向微米级纤维网能够调节细胞形态。为了验证这一点，在产生平均纤维直径为0.14 - 3.6微米、角度标准偏差为31 - 60度的条件下，将聚（D,L - 乳酸 - 羟基乙酸）（PLGA）的融合纤维网静电纺丝到刚性支撑物上。对贴壁的NIH 3T3成纤维细胞形态的分析表明，投影细胞面积和长宽比随着纤维直径和纤维定向程度的增加而系统性地增加。重要的是，3.6微米纤维上的细胞形态与旋涂PLGA薄膜上的相似。最后，静电纺丝网的细胞密度与旋涂PLGA没有显著差异，这表明细胞增殖对纤维直径或定向不敏感。（c）2006爱思唯尔有限公司。保留所有权利。
Engineered ligament tissues are promising materials for the repair of tears and ruptures, but require the development of biomaterial scaffolds that not only support physiologically relevant loads, but also possess architectures capable of orienting cell adhesion and extracellular matrix deposition. Based on evidence that micron-scale topographic features induce cell orientation through a contact guidance phenomenon, we postulate that oriented micron-scale fiber meshes-formed by the electrospinning process-can regulate cell morphology. To test this, fused fiber meshes of poly(D,L-lactic-co-glycolic acid) (PLGA) were electrospun onto rigid supports under conditions that produced mean fiber diameters of 0.14-3.6 mu m, and angular standard deviations of 31-60 degrees. Analysis of the morphology of adherent NIH 3T3 fibroblasts indicated that projected cell area and aspect ratio increased systematically with both increasing fiber diameter and degree of fiber orientation. Importantly, cell morphology on 3.6 mu m fibers was similar to that on spincoated PLGA films, Finally, cell densities on electrospun meshes were not significantly different from spincoated PLGA, indicating that cell proliferation is not sensitive to fiber diameter or orientation. (c) 2006 Elsevier Ltd. All rights reserved.