GM-CSF in the generation of dendritic cells from human blood monocyte precursors: Recent advances
GM-CSF in the generation of dendritic cells from human blood monocyte precursors: Recent advances
复制标题
DOI：
10.1016/j.imbio.2008.07.017
复制
发表时间：
2008-01-01
期刊：
影响因子：
2.8
通讯作者：
Gessani, Sandra
中科院分区：
文献类型：
--
作者：
Conti, Lucia;Gessani, Sandra
树突状细胞 (DC) 凭借其控制免疫和耐受诱导的能力，在免疫系统的协调中发挥着关键作用。 DC 的功能取决于子集及其位置和激活状态。许多体外实验方案旨在重现造血前体细胞的 DC 生成，这些实验表明微环境和细胞因子环境在驱动具有特殊表型和功能特征的 DC 生成中的重要性。最近，有关从人血单核细胞发育 DC 的一些重要概念受到了挑战。研究发现，仅在粒细胞/巨噬细胞集落刺激因子 (GM-CSF) 存在的情况下，单核细胞就可以生成人类 DC，这一发现表明，在具有独特特性的 DC 发育过程中，这种细胞因子是直接参与者，而不是存活促进因子。这篇综述总结了不同细胞因子混合物在体外从血液单核细胞前体生成人类 DC 中的作用，重点关注 GM-CSF 作为功能独特 DC 生成的直接参与者。 (C) 2008 爱思唯尔有限公司。版权所有。
Dendritic cells (DCs) play a key role in the orchestration of the immune system by virtue of their capacity to control both immunity and tolerance induction. The functions of DCs depend on the subset as well as their location and activation state. A number of in vitro protocols, developed to recapitulate DC generation from hematopoietic precursors, have suggested the importance of microenvironment and cytokine milieu in driving the generation of DCs endowed with peculiar phenotypic and functional features. Recently, some important concepts on the development of DCs from human blood monocytes have been challenged. The finding that human DCs can be generated from monocytes in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF) alone points to this cytokine as a direct player, rather than a survival-promoting factor, in the development of DCs with distinct properties.This review summarizes the role of different cytokine cocktails in the in vitro generation of human DCs from blood monocyte precursors, focusing on GM-CSF as a direct player in the generation of functionally distinct DCs. (C) 2008 Elsevier GmbH. All rights reserved.