Chronic intraventricular infusion with NGF improves LTP in old cognitively-impaired rats
Chronic intraventricular infusion with NGF improves LTP in old cognitively-impaired rats
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DOI：
10.1016/s0006-8993(97)00610-0
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发表时间：
1997-10-03
期刊：
影响因子：
2.9
通讯作者：
Gonzalez, O
中科院分区：
文献类型：
--
作者：
Bergado, JA;Fernandez, CI;Gonzalez, O
年长（21 个月）认知障碍雄性 Sprague-Dawley 大鼠使用微渗泵接受神经生长因子 (NGF) 或细胞色素 C (Cit C) 脑室内输注 14 或 28 天，并在治疗后 1 周或 3 个月进行评估。还对未治疗的年轻大鼠、老年受损大鼠和老年未受损大鼠组进行了评估。在麻醉状态下记录和刺激电极被立体定向地植入齿状回和穿通路径中。单独调整刺激强度以获得测试刺激的一半最大群体峰值（P）和强直刺激的四分之一最大群体峰值（P）。在 400 Hz 强直刺激之前和之后 2、5、15、30 和 60 分钟测定 P 的幅度和潜伏期以及场 EPSP 的斜率 (S)。在强直之前和之后也以 30 毫秒的间隔进行配对刺激。老年认知受损大鼠在振幅和潜伏期上均表现出 S 增强缺失和 P 增强延迟，而未受损大鼠的行为与年轻对照组相似。成对脉冲抑制在强直前或强直后各组之间没有显示差异，这表明增强作用受损并非由于追溯抑制增加所致。 NGF 治疗将 LTP 缺陷改善至与未受损大鼠相当的水平，而 Cit C 对照组则没有任何改善。 NGF 治疗组之间没有出现差异，但有证据表明，治疗 3 个月后评估的动物产生了更强的增强作用。 (C) 1997 Elsevier Science B.V.
Aged (21 months) cognitively-impaired male Sprague-Dawley rats received intraventricular infusion of nerve growth factor (NGF) or cytochrome C (Cit C) for 14 or 28 days using miniosmotic pumps and were evaluated either 1 week or 3 months after treatment. Groups of untreated young, aged-impaired and aged non-impaired rats were also evaluated. Under narcose recording and stimulating electrodes were stereotactically implanted in the dentate gyrus and the perforant path. The stimulation intensity was individually adjusted to obtain a half-maximal population spike (P) for test stimuli and a quarter-maximal for tetanization. The amplitude and latency of P and the slope (S) of the field EPSP were determined before and at 2, 5, 15, 30 and 60 min after tetanization at 400 Hz. Paired stimuli at 30 ms interval were also applied before and after tetanization. Aged, cognitively impaired rats showed an absent S potentiation and a delayed P potentiation, both in amplitude and latency, while non-impaired rats behaved like the young controls. Paired pulse inhibition showed no difference among groups before or after tetanization suggesting that the impaired potentiation is not due to an increased retroactive inhibition. NGF treatment ameliorates LTP deficits to levels equivalent to non-impaired rats, while Cit C controls showed no improvement. No differences appear among NGF treated groups, but evidence suggest that the animals evaluated 3 months after treatment developed a stronger potentiation. (C) 1997 Elsevier Science B.V.