Application of microsatellite markers to population genetics studies of Japanese flounder Paralichthys olivaceus
Application of microsatellite markers to population genetics studies of Japanese flounder Paralichthys olivaceus
复制标题
DOI：
10.1007/s10126-001-0064-8
复制
发表时间：
2001-11-01
影响因子：
3
通讯作者：
Hara, M
中科院分区：
文献类型：
--
作者：
Sekino, M;Hara, M
利用微卫星分析技术对日本近海7个牙鲆群体的遗传结构进行了研究。正如预期的那样，所有11个微卫星位点的检测是可变的，在所有的群体（每个位点的等位基因数，15.2-18.2，平均预期杂合性，0.74-0.78）。在21个可能的成对比较中，11个群体对表现出与等位基因频率分布或固定指数（F-ST）相关的显著遗传异质性。改良的Cavalli-Sforza弦距离（D-A）和Nei标准遗传距离（D-ST）分别在0.051 ~ 0.090和0.000 ~ 0.025之间。有证据表明，在这项研究中评估的人口不是来自一个单一的panmictic人口，但是，它似乎在日本各地的日本比目鱼人口没有良好的结构，作为一个估计的固定指数值之间的7个地方是非常低的（F-ST = 0.0025）。
We examined population genetic structure by means of microsatellite analysis among 7 Japanese flounder (Paralichthys olivaceus) populations collected from coastal sea areas around Japan. As was expected, all of the 11 microsatellite loci examined were variable in all populations (number of alleles per locus, 15.2-18.2; average of expected heterozygosity, 0.74-0.78). Eleven population pairs in 21 possible pairwise comparisons showed significant genetic heterogeneity associated with allele frequency distributions or fixation index (F-ST). Modified Cavalli-Sforza chord distance (D-A) and Nei's standard genetic distance (D-ST) ranged from 0.051 to 0.090, and from 0.000 to 0.025, respectively. There was evidence that the populations assessed in this study were not drawn from a single panmictic population; however, it appears that Japanese flounder populations around Japan are not well-structured, as an estimate of the fixation index value among the 7 localities was very low (F-ST = 0.0025).