Cellular events within peripodial epithelia that accompany evagination of Manduca wing discs: Conversion of cuboidal epithelia to columnar epithelia
Cellular events within peripodial epithelia that accompany evagination of Manduca wing discs: Conversion of cuboidal epithelia to columnar epithelia
复制标题
伴随天蛾翼盘外翻的足周上皮内的细胞事件：立方上皮向柱状上皮的转化
DOI：
10.1016/0012-1606(87)90202-8
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
S. M. Magee
中科院分区：
文献类型：
--
作者：
J. Nardi;S. Norby;S. M. Magee
在manduca的翅盘中，一层鞘周上皮完全覆盖在另一层上皮的顶端表面，注定形成翼叶。足周上皮的立方体细胞不仅附着在厚的基板上，而且它们的外侧和基板表面高度卷曲，并有强烈的钌红染色。相反，翅上皮柱状细胞缺乏基底层和rr阳性表面。外翻时，rr阳性物质消失，足周上皮内外侧细胞接触范围增加。与这种侧向“拉链”同时，整个腹膜收缩并滑过翼叶上皮，从而使翅膀暴露在昆虫的外表面。胰蛋白酶治疗下腺组织加速了足周上皮细胞表面rr阳性物质的外翻和消失。显然，腹膜的收缩及其侧细胞接触的增加伴随着其侧细胞表面和基底细胞表面酸性糖蛋白的消失。
In the wing disc ofManduca, a sheet of peripodial epithelium completely covers the apical surface of another epithelium destined to form the wing blade. The cubodial cells of the peripodial epithelium not only are attached to a thick basal lamina but also their lateral and basal surfaces are highly convoluted and stain intensely with ruthenium red (RR). In contrast, the columnar cells of the wing epithelium lack both a basal lamina and RR-positive surfaces. During evagination, the RR-positive material disappears and the extent of lateral cell contact within the peripodial epithelium increases. Concurrently with this lateral “zippering”, the entire peripodial sheet contracts and slides over the wing blade epithelium, thereby exposing the wing to the external surface of the insect. Trypsin treatment ofManducadiscs accelerates both evagination and the disappearance of RR-positive material from the surfaces of cells in the peripodial epithelium. Apparently contraction of the peripodial sheet and the increase in its lateral cell contacts is accompanied by the disappearance of acidic glycoproteins from its lateral and basal cell surfaces.