1H nuclear magnetic resonance-based plasma metabolomics provides another perspective of response mechanisms of newborn calves upon the first colostrum feeding
1H nuclear magnetic resonance-based plasma metabolomics provides another perspective of response mechanisms of newborn calves upon the first colostrum feeding
复制标题
基于H-1核磁共振的血浆代谢组学为新生犊牛第一次初乳喂养的反应机制提供了另一个视角
DOI：
10.1093/jas/sky078
复制
发表时间：
2018-05-01
影响因子：
3.3
通讯作者：
Tao, J. Z.
中科院分区：
文献类型：
--
作者：
Guo, Y. S.;Tao, J. Z.
新生小牛出生后的第一餐对生存和健康至关重要。新生犊牛第一次饲喂初乳后，血液IgG水平显著升高。然而，当时关于新生小牛血液小分子代谢物的全面信息很少。在这项研究中，新生犊牛的血浆代谢产物的变化后，最初的初乳喂养，首次全面的H-1核磁共振（NMR）检查。用1H-1 NMR分析了8头犊牛饲喂前后的16个血浆样品。多变量分析显示代谢谱存在显著差异。进食后，急性期N-乙酰化糖蛋白13其他血浆代谢产物减少，而19血浆代谢产物增加。对这些代谢物的代谢组学途径分析表明，新生小牛中包括脂质、碳水化合物和氨基酸代谢在内的13种代谢途径的改变反映了首次饲喂初乳时的总体代谢反应。这些结果表明，除了满足能量需求，4.0 L的高品质初乳喂养出生后4小时内有积极的作用，缓解新生犊牛的产后应激。本研究从另一个角度探讨了新生犊牛对第一次初乳喂养的反应机制。
The first meal of a neonatal calf after birth is crucial for survival and health. Blood IgG levels remarkably increase in neonatal calves after the first colostrum feeding. However, there is little comprehensive information on blood small-molecule metabolites in neonatal calves at that time. In this study, the changes in plasma metabolites of neonatal calves after the initial colostrum feeding were first examined with comprehensive H-1 nuclear magnetic resonance (NMR). Sixteen plasma samples obtained from 8 calves before and after feeding were analyzed with H-1 NMR. Multivariate analyses revealed a significant difference in metabolic profiles. After feeding, acute phase N-acetylated glycoproteins 13 other plasma metabolites decreased, whereas 19 plasma metabolites increased. Metabolomics pathway analysis of these metabolites revealed that a global metabolic response on the first colostrum feeding was reflected by alterations of 13 metabolic pathways including lipid, carbohydrate, and amino acid metabolism in neonatal calves. These results suggested that besides meeting energy demand, a 4.0 L of high-quality colostrum feeding within 4 h after birth had a positive effect on relieving the postnatal stress in neonatal calves. This study provides another perspective of response mechanisms of newborn calves upon the first colostrum feeding.