Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
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DOI：
10.3791/57731
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发表时间：
2018-09-01
影响因子：
1.2
通讯作者：
Vermulst, Marc
中科院分区：
文献类型：
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作者：
Fritsch, Clark;Gout, Jean-Francois Pierre;Vermulst, Marc
准确的转录是基因信息忠实表达所必需的。然而，令人惊讶的是，人们对控制转录保真度的机制知之甚少。为了填补这一科学知识空白，我们最近优化了环测序方法，以检测酿酒酵母、黑腹果蝇和秀丽线虫的转录组中的转录错误。这一协议将为研究人员提供一个强大的新工具来绘制真核细胞中转录错误的图景，以便可以前所未有的详细地阐明控制转录保真度的机制。
Accurate transcription is required for the faithful expression of genetic information. Surprisingly though, little is known about the mechanisms that control the fidelity of transcription. To fill this gap in scientific knowledge, we recently optimized the circle-sequencing assay to detect transcription errors throughout the transcriptome of Saccharomyces cerevisiae, Drosophila melanogaster, and Caenorhabditis elegans. This protocol will provide researchers with a powerful new tool to map the landscape of transcription errors in eukaryotic cells so that the mechanisms that control the fidelity of transcription can be elucidated in unprecedented detail.