DNA repair in the metallothionein gene increases with transcriptional activation.
DNA repair in the metallothionein gene increases with transcriptional activation.
复制标题
金属硫蛋白基因中的 DNA 修复随着转录激活而增加。
DOI：
10.1093/nar/15.23.10021
复制
发表时间：
1987
影响因子：
14.9
通讯作者：
Bohr,VA
中科院分区：
文献类型：
--
作者：
Okumoto,DS;Bohr,VA
我们研究了中国仓鼠卵巢(CHO)金属硫蛋白(MT)基因在紫外光损伤后的DNA修复。在有或没有基因转录激活的情况下，通过在重金属氯化锌存在的情况下孵育来比较修复情况。而未诱导状态下修复效率很低，基因诱导后修复效率显著提高。氯化锌的存在似乎没有改变细胞中的其他修复参数。无论基因是否被诱导，紫外光照射后的基因组DNA修复效率是相似的，我们先前描述的必需二氢叶酸还原酶(DHFR)基因的优先DNA修复模式不受氯化锌的存在的影响。根据对两个含有MT I基因的不同限制性内切酶片段的修复分析，我们得出结论：诱导后的有效修复区域比基因的1kb大得多。结果表明，基因组区域对DNA修复酶的可及性可能受到局部染色质结构的动态调节。
We have studied DNA repair in the Chinese Hamster Ovary (CHO) metallothionein (MT) gene after UV-light induced damage. The repair was examined comparatively with or without transcriptional activation of the gene by incubation in the presence of the heavy metal ZnCl2. Whereas the repair efficiency was very low in the uninduced state, it increased significantly after induction of the gene. The presence of ZnCl2did not appear to change other repair parameters in the cells. The overall genome DNA repair efficiency after UV irradiation was similar whether or not the gene was induced and the preferential DNA repair pattern in the essential dihydrofolate reductase (DHFR) gene which we have previously described was unaffected by the presence of ZnCl2. Based upon repair analysis in two different restriction fragments containing the MT I gene, we conclude that the region of efficient repair after induction is considerably larger than the 1 kb size of the gene. The results suggest that the accessibility of a genomic region to DNA repair enzymes may be regulated by the local chromatin structure in a dynamic manner.