Novel probiotic approach to counter Paenibacillus larvae infection in honey bees
Novel probiotic approach to counter Paenibacillus larvae infection in honey bees
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DOI：
10.1038/s41396-019-0541-6
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发表时间：
2020-02-01
期刊：
影响因子：
11
通讯作者：
Reid, Gregor
中科院分区：
文献类型：
--
作者：
Daisley, Brendan A.;Pitek, Andrew P.;Reid, Gregor
美洲蜜蜂幼虫病（American foulbrood，AFB）是一种危害意大利蜜蜂（Apis mellifera）的高致病性疾病。病原体类芽孢杆菌幼虫攻击蜜蜂育雏，并使整个蜂箱在活跃的疾病状态下功能失调，但更常见的是无症状地作为不活跃的孢子居住在蜂箱中，甚至躲避警惕的养蜂人。这种致病转变的机制尚未完全清楚，AFB也没有治愈方法。在这里，我们评估了蜂箱补充益生菌乳酸杆菌（通过营养肉饼; BioPatty）如何影响菌落对自然发生的AFB爆发的抵抗力。结果表明，BioPatty处理过的蜂箱中蜜蜂幼虫的病原体负荷和蛋白水解活性显着降低。有趣的是，在监测期间，无论处理组如何，成年保育蜂的微生物群组成都发生了明显的变化，但只有补充媒介物的保育蜂表现出更高的P.幼虫负荷。利用实验室饲养的蜜蜂幼虫进行的体外实验表明，植物乳杆菌Lp 39，鼠李糖乳杆菌GR-1和昆氏乳杆菌BR-1（包含在BioPatty中）可以减少病原体负荷，上调关键免疫基因的表达，并提高幼虫感染期间的存活率。这些发现表明，使用一种对养蜂人来说实用且负担得起的含有乳酸杆菌的蜂箱补充剂，可能有效减少与地方病病原体相关的蜂箱损失。
American foulbrood (AFB) is a highly virulent disease afflicting honey bees (Apis mellifera). The causative organism, Paenibacillus larvae, attacks honey bee brood and renders entire hives dysfunctional during active disease states, but more commonly resides in hives asymptomatically as inactive spores that elude even vigilant beekeepers. The mechanism of this pathogenic transition is not fully understood, and no cure exists for AFB. Here, we evaluated how hive supplementation with probiotic lactobacilli (delivered through a nutrient patty; BioPatty) affected colony resistance towards a naturally occurring AFB outbreak. Results demonstrated a significantly lower pathogen load and proteolytic activity of honey bee larvae from BioPatty-treated hives. Interestingly, a distinctive shift in the microbiota composition of adult nurse bees occurred irrespective of treatment group during the monitoring period, but only vehicle-supplemented nurse bees exhibited higher P. larvae loads. In vitro experiments utilizing laboratory-reared honey bee larvae showed Lactobacillus plantarum Lp39, Lactobacillus rhamnosus GR-1, and Lactobacillus kunkeei BR-1 (contained in the BioPatty) could reduce pathogen load, upregulate expression of key immune genes, and improve survival during P. larvae infection. These findings suggest the usage of a lactobacilli-containing hive supplement, which is practical and affordable for beekeepers, may be effective for reducing enzootic pathogen-related hive losses.