Identification of N-acetyl Proline-Glycine-Proline (acPGP) in human serum of adults and newborns by liquid chromatography-tandem mass spectrometry.
Identification of N-acetyl Proline-Glycine-Proline (acPGP) in human serum of adults and newborns by liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/j.cca.2008.12.031
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
A. Shibata;I. Morioka;Chitose Ashi;S. Nagasaki;C. Tode;S. Morikawa;A. Miwa;M. Enomoto;K. Saiki;N. Yokoyama;A. Takeuchi;M. Matsuo
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作者：
A. Shibata;I. Morioka;Chitose Ashi;S. Nagasaki;C. Tode;S. Morikawa;A. Miwa;M. Enomoto;K. Saiki;N. Yokoyama;A. Takeuchi;M. Matsuo
乙酰基脯氨酸-甘氨酸-脯氨酸（acPGP）是一种新型的中性粒细胞化学引诱物。然而，还没有研究报告，以确定存在的acPGP在人血清中。本研究旨在建立一种检测人血清中acPGP的方法，并探讨其在人血清中的存在情况。采用液相色谱-串联质谱（LC-MS/MS）多反应监测（MRM）正离子模式，对acPGP进行了灵敏分析，其主要产物离子（[M+H]+离子，m/z 312）出现在112和140。人血清中的MRM（转换：m/z 312/112）色谱图显示与真实acPGP保留时间相同的单峰。acPGP标准品的线性关系良好，定量结果具有较高的精密度。成人和新生儿的平均血清acPGP水平分别为6.3和18.7 pg/ml。在新生儿中，低出生体重儿有显着较高的血清acPGP levels.CONCLUSIONSWe建立了一种方法，用于血清acPGP的定量使用LC-MS/MS，本文提供了第一个证据acPGP在成人和新生儿的人血清中的存在。
BACKGROUNDN-acetyl Proline–Glycine–Proline (acPGP) is a novel neutrophil chemoattractant. However, no studies have been reported to identify the presence of acPGP in human serum. The purpose of our study was to establish a method for measuring acPGP, and to determine whether acPGP is present in human serum.METHODSSerum samples were obtained from 22 healthy adults and 26 term and preterm newborns. For the sensitive analysis of acPGP, we utilized liquid chromatography–tandem mass spectrometry (LC–MS/MS) with a multiple reaction monitoring (MRM) positive ion mode.RESULTSThe major product ions of acPGP ([M+H]+ion, m/z 312) appeared at 112 and 140. The MRM (transition: m/z 312/112) chromatogram in human serum showed a single peak with the same retention time as that of authentic acPGP. The calibration curve of authentic acPGP was linear, and our quantitative results indicated high precision. The mean serum acPGP levels in adults and newborns were 6.3 and 18.7 pg/ml, respectively. In newborns, lower birth weight infants had significantly higher serum acPGP levels.CONCLUSIONSWe established a method for the quantification of serum acPGP using LC–MS/MS, and this paper provides the first evidence for the presence of acPGP in human serum of adults and newborns.