The major-effect QTL CsARN6.1 encodes an AAA-ATPase domain-containing protein that is associated with waterlogging stress tolerance through promoting adventitious root formation
The major-effect QTL CsARN6.1 encodes an AAA-ATPase domain-containing protein that is associated with waterlogging stress tolerance through promoting adventitious root formation
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主效应 QTL CsARN6.1 编码一种含有 AAA-ATPase 结构域的蛋白质，该蛋白质通过促进不定根形成与耐涝胁迫相关
DOI：
10.1111/tpj.13819
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通讯作者：
Xuehao Chen
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作者：
Xuewen Xu;Jing Ji;Qiang Xu;Xiaohua Qi;Yiqun Weng;Xuehao Chen
In plants, the formation of hypocotyl-derived adventitious roots (AR) is an important morphological acclimation to waterlogging stress; however, its genetic basis remains fragmentary. Here, through combined use of bulked segregant analysis-based whole genome sequencing, SNP haplotyping and fine genetic mapping, we identified a candidate gene for a major-effect QTL ARN6.1 that was responsible for waterlogging tolerance due to increased AR formation in the cucumber line Zaoer-N. Through multiple lines of evidence, we show that the CsARN6.1 was the most possible candidate for ARN6.1 which encodes an AAA ATPase. The increased AR formation under waterlogging in Zaoer-N could be attributed to a non-synonymous SNP in the coiled-coil domain region of this gene. CsARN6.1 increases the AR numbers via its ATPase activity. Ectopic expression of CsARN6.1 in Arabidopsis resulted in better rooting ability and lateral root development in the transgenic plants. Transgenic cucumber expressing CsARN6.1Asp allele from Zaoer-N exhibited significantly increase in AR numbers than the wild type expressing the allele same to Pepino under waterlogging condition. Taken together, these data support this AAA ATPase gene CsARN6.1 as an important player in increasing cucumber AR formation and waterlogging tolerance.
