JOINT REPORT OF THE THIRD INTERNATIONAL WORKSHOP ON CANINE IMMUNOGENETICS: II. ANALYSIS OF THE SEROLOGICAL TYPING OF CELLS
JOINT REPORT OF THE THIRD INTERNATIONAL WORKSHOP ON CANINE IMMUNOGENETICS: II. ANALYSIS OF THE SEROLOGICAL TYPING OF CELLS
复制标题
第三届犬免疫遗传学国际研讨会联合报告：II．
作者：
R. W. Bull;H. Vriesendorp;R. Cech;H. Grosse;A. Bijma;W. Ladiges;K. Krumbacher;I. Doxiadis;H. Ejima;J. Templeton;E. Albert;R. Storb;H. Deeg
采用标准化的微淋巴细胞毒性试验，七个国际实验室评估了319只杂交犬和152只比格犬的144种抗犬淋巴细胞抗原（DLA）血清。研讨会确认了DLA-A2、A3、A9的血清学定义；Dla-b4, b5, b6, b13；DLA-C11 (Cwl);和C12 (Cw2)。两个新的特异性被分配到DLA-A位点（aw14和aw15），只有在混合品种的狗。第三个特异性（Cw3）被分配到DLA-C位点。这些等位基因的抗原和基因频率在两组狗之间有所不同，但“空白”的频率在两组狗中相似。未来的国际合作将有必要更全面地确定狗的主要组织相容性复合体（MHC）的多态性。这些努力将受益于本次研讨会建立的标准血清学检测。
Using a standardized microlymphocytotoxicity assay, seven international laboratories evaluated 144 anti-dog lymphocyte antigen (DLA) sera in 319 mixed breed and 152 Beagle dogs. The workshop confirmed the serological definitions for DLA-A2, A3, A9; DLA-B4, B5, B6, B13; DLA-C11(Cwl); and C12(Cw2). Two new specificities were assigned to the DLA-A locus (Awl4 and Aw 15) in only the mixed breed dogs. A third specificity (Cw3), was assigned to the DLA-C locus. The antigen and gene frequencies of these alleles differed between the two groups of dogs, but the frequencies of the “blank” were similar in both groups. Future international collaborations will be necessary to definite more completely the polymorphisms of the major histocompatibility complex (MHC) of the dog. Those efforts will benefit from the standard serological test established in this workshop.