Neural activity and branching of embryonic retinal ganglion cell dendrites
Neural activity and branching of embryonic retinal ganglion cell dendrites
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DOI：
10.1016/j.mod.2012.05.003
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发表时间：
2012-07-01
影响因子：
2.6
通讯作者：
McFarlane, S.
中科院分区：
文献类型：
--
作者：
Hocking, J. C.;Pollock, N. S.;McFarlane, S.
神经元树突的形状对其功能至关重要，因为它决定了神经元可以接收的输入数量以及这些输入是如何处理的。在发育过程中，神经元启动初级树突，这些树突分枝形成简单的树枝。随后，生长通过一个过程发生，这个过程结合了现有树枝的伸展和收缩，以及新分支的增加。视网膜神经节细胞(RGC)细小终末分支的丢失和增加依赖于其突触伙伴--无长突细胞和双极细胞的传入输入。然而，尚不清楚神经活动是否调节初级树突的启动及其最初的分支。为了研究这一点，在体内发育的非洲爪哇视网膜节细胞表达延迟整流型电压门控钾(KV)通道非洲爪哇Kv1.1或人类内向整流通道Kir2.1，以调节非洲爪哇脊髓神经元的电活动。任何一种钾通道的错误表达都增加了分支点的数量和所有分支的总长度。结果表明，表达绿色荧光蛋白的RGCs和异位表达高度相关的突变型非功能Kv1.1通道的RGCs的树突状突起明显大于对照RGCs。我们的数据表明，膜兴奋性调节RGC树突状乔木的最早分化。(C)2012爱思唯尔爱尔兰有限公司。保留所有权利。
The shape of a neuron's dendritic arbor is critical for its function as it determines the number of inputs the neuron can receive and how those inputs are processed. During development, a neuron initiates primary dendrites that branch to form a simple arbor. Subsequently, growth occurs by a process that combines the extension and retraction of existing dendrites, and the addition of new branches. The loss and addition of the fine terminal branches of retinal ganglion cells (RGCs) is dependent on afferent inputs from its synaptic partners, the amacrine and bipolar cells. It is unknown, however, whether neural activity regulates the initiation of primary dendrites and their initial branching. To investigate this, Xenopus laevis RGCs developing in vivo were made to express either a delayed rectifier type voltage-gated potassium (KV) channel, Xenopus Kv1.1, or a human inward rectifying channel, Kir2.1, shown previously to modulate the electrical activity of Xenopus spinal cord neurons. Misexpression of either potassium channel increased the number of branch points and the total length of all the branches. As a result, the total dendritic arbor was bigger than for control green fluorescent protein-expressing RGCs and those ectopically expressing a highly related mutant non-functional Kv1.1 channel. Our data indicate that membrane excitability regulates the earliest differentiation of RGC dendritic arbors. (C) 2012 Elsevier Ireland Ltd. All rights reserved.