Full sequence analysis of the original Sapporo virus
Full sequence analysis of the original Sapporo virus
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DOI：
10.1111/j.1348-0421.2011.00358.x
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发表时间：
2011-09-01
影响因子：
2.6
通讯作者：
Tsutsumi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Nakanishi, Kaori;Tatsumi, Masatoshi;Tsutsumi, Hiroyuki
在本研究中，鉴定了沙波病毒原型，即札幌病毒（SV 82）的全长基因组序列。从粪便样品中提取札幌病毒RNA，通过RT-PCR扩增，PCR产物直接测序并分析。序列分析表明，札幌病毒全长7433个核苷酸，具有3个开放阅读框。札幌株与曼彻斯特病毒的核苷酸序列一致性为91.7%。系统发育分析还揭示了札幌病毒与其他Sapovirus/基因组I毒株的接近性。这里提供了关于sapovirus分析进化历史的基本信息。
In this study, the full-length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT-PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo virus consists of 7433 nucleotides and has three open reading frames. The Sapporo strain shows 91.7% nucleotide sequence identity to the Manchester virus. Phylogenic analysis has also revealed the closeness of Sapporo virus to other sapovirus/genogroup I strains. Basic information on the evolutionary history of sapovirus analysis is provided here.