[Study on influence of arbuscular mycorrhizal fungi Pinellia ternata yield and chemical composition].

[Study on influence of arbuscular mycorrhizal fungi Pinellia ternata yield and chemical composition].
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DOI:
10.4268/cjcmm20100317
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发表时间:
2010-02
期刊:
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子:
--
通讯作者:
Q. Guo;Li-tao Cheng;Zuoyi Liu
Q. Guo;Li-tao Cheng;Zuoyi Liu
中科院分区:
其他
文献类型:
--
作者:
Q. Guo;Li-tao Cheng;Zuoyi Liu

文献摘要

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目的研究丛枝菌根真菌对半夏叶指数、繁殖系数、块茎产量及化学成分的影响。方法在生长过程中直至半夏倒伏,测量半夏叶长、叶宽、株高和叶柄直径。收获后计算叶片指数,测定块茎产量。采用高效液相色谱法测定鸟苷的含量,分光光度法测定生物碱的含量。结果与结论接种AM真菌可提高半夏的繁殖系数和块茎产量。接种AM真菌可提高半夏叶片指数,使叶片更趋向于竹叶型。接种AM真菌能延缓半夏倒伏时间; mosseae + G.根内处理(早诱导期)对提高半夏块茎鲜重、干重和繁殖系数效果较好。接种AM真菌能提高半夏块茎中鸟苷和生物碱的含量,且接种处理的鸟苷和生物碱含量均高于赫章栽培半夏; mosseae + G.根内处理(接种后)对提高鸟苷和生物碱含量有较好的效果。
OBJECTIVE To study the influence of arbuscular mycorrhizal fungi to Pinellia ternata leaf index, propagation coefficient, tuber yield and chemical composition. METHOD The Pinellia ternata leaf length, width, plant height and leafstalk diameter were measured during growing time till P. ternata loded. The leaf index was calculated and the tuber yield were measured after harvest. The contents of guanosine was determined by HPLC method, and the alkaloid contents were determined by spectrophotometric method. RESULT AND CONCLUSION Inoculated AM fungi could increase P. ternata propagation coefficient and the yield of tuber. Inoculated AM fungi could increase P. ternata leaf index, made leaf tends to be more like bamboo leaf type. Inoculated AM fungi could delayed P. ternata lodging time; G. mosseae + G. intraradices treatment (early inolulation time) proved better influence on increasing fresh weight and dry weight of P. ternata tuber and the propagation coefficient. Inoculated AM fungi could increase guanosine and alkaloid contents of Pinellia ternata tuber, and the contents of guanosine and alkaloid in inoculated treatments were higher than those in cultivated P. ternata from Hezhang; G. mosseae + G. intraradices treatment (later inolulation time) proved good effect on increasing the contents of guanosine and alkaloid.