ORYZA SATIVA MYOSIN XI B controls pollen development by photoperiod-sensitive protein localizations
ORYZA SATIVA MYOSIN XI B controls pollen development by photoperiod-sensitive protein localizations
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DOI：
10.1016/j.ydbio.2007.01.008
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发表时间：
2007-04-15
影响因子：
2.7
通讯作者：
Ramachandran, Srinivasan
中科院分区：
文献类型：
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作者：
Jiang, Shu-Ye;Cai, Minnie;Ramachandran, Srinivasan
肌球蛋白是肌动蛋白为基础的运动蛋白，负责各种运动和信号转导。只有一小部分肌球蛋白类存在于植物中，对它们的功能知之甚少。在这里，我们展示了水稻肌球蛋白基因如何通过感知变化的环境因子来控制花粉发育。该分析是基于基因捕获Ds插入突变体水稻肌球蛋白XI B（osmyoXIB）。该突变体在短日照（SD）条件下表现雄性不育，在长日照（LD）条件下表现可育。在SD和LD条件下，在整个花药中检测到OSMYOXIB转录物。然而，在SD条件下，OSMYOXIB-GUS融合蛋白仅定位于花药的表皮层中，这是由于缺少3 '-非翻译区（3'-UTR）并且在Ds插入之后稀释（DIL）结构域序列。结果，突变体花粉发育受到影响，导致雄性不育。而在LD条件下，融合蛋白在花药中定位正常。尽管定位正常，但由于缺乏DIL结构域序列，该蛋白质仅具有部分功能，导致花粉育性的恢复有限。这项研究还为基因表达的新分子方面提供了一个案例，即，花药中细胞层特异性翻译。(c)2007爱思唯尔公司All rights reserved.
Myosins are actin-based motor proteins responsible for various motility and signal transduction. Only a small set of myosin classes is present in plants, and little is known about their functions. Here we showed how a rice myosin gene controlled pollen development by sensing changed environmental factors. The analysis is based on a gene-trapped Ds insertion mutant Oryza sativa myosin XI B (osmyoXIB). This mutant showed male sterility under short day length (SD) conditions and fertility under long day length (LD) conditions. Under both SD and LD conditions, the OSMYOXIB transcript was detected in whole anthers. However, under SD conditions, the OSMYOXIB-GUS fusion protein was localized only in the epidermal layer of anthers due to the lack of 3'-untranslated region (3'-UTR) and to dilute (DIL) domain sequences following the Ds insertion. As a result, mutant pollen development was affected, leading to male sterility. By contrast, under LD conditions, the fusion protein was localized normally in anthers. Despite normal localization, the protein was only partially functional due to the lack of DIL domain sequences, resulting in limited recovery of pollen fertility. This study also provides a case for a novel molecular aspect of gene expression, i.e., cell layer-specific translation in anthers. (c) 2007 Elsevier Inc. All rights reserved.