Comparative molecular cytogenetic studies in the order Carnivora: mapping chromosomal rearrangements onto the phylogenetic tree
Comparative molecular cytogenetic studies in the order Carnivora: mapping chromosomal rearrangements onto the phylogenetic tree
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DOI：
10.1159/000063032
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发表时间：
2002-01-01
影响因子：
1.7
通讯作者：
Ferguson-Smith, MA
中科院分区：
文献类型：
--
作者：
Graphodatsky, AS;Yang, F;Ferguson-Smith, MA
我们通过流式分选染色体的简并寡核苷酸引物 PCR (DOP-PCR) 扩增，为美国水貂制作了一套染色体特异性绘画探针。这些绘画探针用于界定人类、红狐、狗、猫和鼬科的八种物种之间的同源染色体片段，包括欧洲水貂、草原和森林鸡貂、黄鼠狼、山黄鼠狼、日本黑貂、条纹鸡貂和獾。根据染色体涂色和G显带的结果，建立了这些物种之间的比较图谱。综合图谱显示了鼬类物种的高水平核型保存。对鼬科动物和外类群物种之间保守染色体片段的比较分析揭示了 18 个假定的祖先常染色体片段，这些片段可能代表了鼬科最近祖先的核型中的祖先染色体或染色体臂。所提出的 2n = 38 祖先鼬科核型似乎已保留在一些现代鼬科动物中，例如貂、路特拉、ktonyx 和 Vormela。鼬科核型从假定的祖先状态的衍生是由于中心融合、裂变、异色臂的添加以及偶尔的中心周倒位。我们的结果证实了其他数据提出的许多进化结论，并通过纳入全基因组染色体重排强化了食肉动物系统发育树的拓扑结构。版权所有 (C) 2002 S. KargerAG，巴塞尔。
We have made a set of chromosome-specific painting probes for the American mink by degenerate oligonucleotide primed-PCR (DOP-PCR) amplification of flow-sorted chromosomes. The painting probes were used to delimit homologous chromosomal segments among human, red fox, dog, cat and eight species of the family Mustelidae, including the European mink, steppe and forest polecats, least weasel, mountain weasel, Japanese sable, striped polecat, and badger. Based on the results of chromosome painting and G-banding, comparative maps between these species have been established. The integrated map demonstrates a high level of karyotype conservation among mustelid species. Comparative analysis of the conserved chromosomal segments among mustelids and outgroup species revealed 18 putative ancestral autosomal segments that probably represent the ancestral chromosomes, or chromosome arms, in the karyotype of the most recent ancestor of the family Mustelidae. The proposed 2n = 38 ancestral Mustelidae karyotype appears to have been retained in some modern mustelids, e.g., Martes, Lutra, ktonyx, and Vormela. The derivation of the mustelid karyotypes from the putative ancestral state resulted from centric fusions, fissions, the addition of heterochromatic arms, and occasional pericentric inversions. Our results confirm many of the evolutionary conclusions suggested by other data and strengthen the topology of the carnivore phylogenetic tree through the inclusion of genome-wide chromosome rearrangements. Copyright (C) 2002 S. KargerAG, Basel.