Antagonistic effects of two herbs in Zuojin Wan, a traditional Chinese medicine formula, on catecholamine secretion in bovine adrenal medullary cells
Antagonistic effects of two herbs in Zuojin Wan, a traditional Chinese medicine formula, on catecholamine secretion in bovine adrenal medullary cells
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DOI：
10.1016/j.phymed.2009.10.010
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发表时间：
2010-07-01
期刊：
影响因子：
7.9
通讯作者：
Gao, X. -M.
中科院分区：
文献类型：
--
作者：
Zhao, F. -R.;Mao, H-P;Gao, X. -M.
为了研究疗效优越、副作用较小的目标，中药组合在植物疗法中的应用在中国和其他一些国家已有数千年的历史。左金丸（ZJW）是一种著名的中药配方，在中国用于治疗胃病。它由黄连（RC）和吴茱萸（FE）两种草药按6：1（w/w）的比例组成。在本研究中，我们研究了 ZJW、RC、FE 以及从这些草药中分离出的活性成分对培养的牛肾上腺髓质细胞中儿茶酚胺 (CA) 分泌和细胞内钙 ([Ca2+](i)) 的影响。 ZJW 和 RC 的提取物以及 RC 的成分小檗碱、巴马汀和药根碱均抑制乙酰胆碱 (ACh)、藜芦定 (Vet) 和/或 56 mM K+ 诱导的 CA 分泌和 [Ca2+](i) 升高。另一方面，FE 的提取物、吴茱萸碱和吴茱萸碱（FE 的成分）刺激 CA 分泌，并由 ACh 诱导 [Ca2+](i) 上升。此外，不同比例的RC和FE引起CA分泌的不同反应。目前的研究结果表明，ZJW中的两种草药对培养的牛肾上腺髓质细胞中乙酰胆碱诱导的CA分泌具有相反的作用，即RC的抑制作用和FE的刺激作用。 (C) 2009 爱思唯尔有限公司。版权所有。
In order to research the target of superior efficacy and lesser side effects, combination of herbal materials has been applied to phytotherapy for thousands of years in China and some other countries. Zuojin Wan (ZJW), a famous traditional Chinese medicine formula, is used in treating gastric diseases in China. It is composed of two herbs, Rhizoma Coptidis (RC) and Fructus Evodiae (FE) in the ratio of 6: 1(w/w). In the present study, we examined the effects of ZJW, RC, FE and active components isolated from these herbs on catecholamine (CA) secretion and intracellular calcium ([Ca2+](i)) in cultured bovine adrenal medullary cells. Extracts of ZJW and RC and berberine, palmatine and jatrorrhizine, components of RC, all inhibited CA secretion and rise in [Ca2+](i) induced by acetylcholine (ACh), veratridine (Vet) and/or 56 mM K+. On the other hand, extract of FE, evodiamine and rutaecarpine, components of FE, stimulated CA secretion and rise in [Ca2+](i) induced by ACh. Furthermore, different proportions of RC and FE caused different responses in CA secretion. The present findings suggest that two herbs in ZJW have opposite effects, i.e., inhibitory effect of RC and stimulatory effect of FE, on CA secretion induced by acetylcholine in cultured bovine adrenal medullary cells. (C) 2009 Elsevier GmbH. All rights reserved.