Diurnal and nocturnal rodents show rhythms in orexinergic neurons
Diurnal and nocturnal rodents show rhythms in orexinergic neurons
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DOI：
10.1016/s0006-8993(02)03264-x
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发表时间：
2002-11-15
期刊：
影响因子：
2.9
通讯作者：
Nunez, AA
中科院分区：
文献类型：
--
作者：
Martínez, GS;Smale, L;Nunez, AA
食欲素 (ORX) A 和 B（下丘脑分泌素）是由外侧下丘脑神经元产生的兴奋性神经肽，与睡眠-觉醒周期的调节有关。在大鼠中，Fos（cfos 基因的产物）表达在涉及睡眠和清醒的区域显示出每日节律，并且食欲素能神经元在夜间显示出 Fos 表达升高。本研究直接比较了白天活动（尼罗罗非鱼；草鼠）和夜间活动（褐家鼠；实验室大鼠）啮齿类动物食欲素能神经元中 Fos 表达的日常模式。保持 12:12 明暗周期的动物在六个不同的 Zeitgeber 时间 (ZT) 进行灌注，在 ZT 0: 1、5、13、17、20 和 23 时亮灯。在夜间和昼间啮齿动物中，食欲素能神经元在 Fos 表达中表现出节律，在每个物种的活跃阶段观察到更多的 Fos。在昼夜动物中，不产生 ORX 的外侧下丘脑细胞中的 Fos 表达在 ZT 20（这些动物睡眠的时间）时升高，而在 ZT 13（活动高峰时间）时表达较低。这些结果为食欲素能神经元的活动与觉醒之间的联系提供了进一步的证据，并且在草鼠中，外侧下丘脑的其他神经元可能以相对于食欲素能神经元的拮抗方式发挥作用，以调节这种昼夜物种的觉醒。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Orexin (ORX) A and B (hypocretins) are excitatory neuropeptides produced by neurons of the lateral hypothalamus that have been implicated in the regulation of the sleep-wake cycle. In rats, Fos (the product of the cfos gene) expression shows daily rhythms in areas involved in sleep and wakefulness and orexinergic neurons show elevated Fos expression during the night. The present study directly compared the daily pattern of Fos expression in orexinergic neurons in diurnal (A. niloticus; grass rats) and nocturnal (R. norvegicus; lab rats) rodents. Animals kept on a 12:12 light-dark cycle were perfused at six different Zeitgeber times (ZT), with lights on at ZT 0: 1, 5, 13, 17, 20 and 23. In both nocturnal and diurnal rodents orexinergic neurons showed rhythms in Fos expression, with more Fos seen during the active phase of each species. In the diurnal species, Fos expression in cells of the lateral hypothalamus that do not produce ORX was elevated at ZT 20, a time when these animals sleep, and was low at ZT 13, a time of peak of activity. These results provide further evidence for a link between activity in orexinergic neurons and wakefulness and that in grass rats, other neurons of the lateral hypothalamus may work in an antagonistic fashion with respect to orexinergic neurons to regulate wakefulness in this diurnal species. (C) 2002 Elsevier Science B.V. All rights reserved.