Safety assessment of Staphylococcus phages of the family Myoviridae based on complete genome sequences.
Safety assessment of Staphylococcus phages of the family Myoviridae based on complete genome sequences.
复制标题
基于完整基因组序列的肌病毒科葡萄球菌噬菌体的安全性评估
DOI：
10.1038/srep41259
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发表时间：
2017-01-24
影响因子：
4.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Cui Z;Guo X;Dong K;Zhang Y;Li Q;Zhu Y;Zeng L;Tang R;Li L
肌病毒科葡萄球菌噬菌体宿主范围广，在噬菌体治疗中具有潜在的应用前景。在本报告中，这些噬菌体的安全性评估是基于它们的完整基因组序列进行的。分析了Myoviridae家族葡萄球菌噬菌体的全基因组，并使用BLAST算法将开放阅读框（Open Reading Frame, orf）与毒力和耐药基因库进行了比较。此外，PHACTS还证实了噬菌体的生活方式（强毒或温和）。生物信息学分析结果显示，所有噬菌体均为裂解型，不含抗性或毒力基因，排除了作为这些不良基因传播载体的可能性。这些发现表明噬菌体在基因组水平上是安全的。sced样转糖基化酶广泛分布于噬菌体基因组中，是万古霉素中间菌株的生物标志物。噬菌体基因组中编码的大约70%的orf功能未知；因此，它们在金黄色葡萄球菌的抗生素耐药性和毒力中的作用尚不清楚，在噬菌体治疗中使用前需要考虑。
Staphylococcus phages of the Myoviridae family have a wide host range and potential applications in phage therapy. In this report, safety assessments of these phages were conducted based on their complete genome sequences. The complete genomes of Staphylococcus phages of the Myoviridae family were analyzed, and the Open Reading Frame (ORFs) were compared with a pool of virulence and antibiotic resistance genes using the BLAST algorithm. In addition, the lifestyle of the phages (virulent or temperate) was also confirmed using PHACTS. The results showed that all phages were lytic and did not contain resistance or virulence genes based on bioinformatic analyses, excluding the possibility that they could be vectors for the dissemination of these undesirable genes. These findings suggest that the phages are safe at the genome level. The SceD-like transglycosylase, which is a biomarker for vancomycin-intermediate strains, was widely distributed in the phage genomes. Approximately 70% of the ORFs encoded in the phage genomes have unknown functions; therefore, their roles in the antibiotic resistance and virulence of Staphylococcus aureus are still unknown and require consideration before use in phage therapy.