Body Weight Loss After Myocardial Infarction in Rats as a Marker of Early Heart Failure Development
Body Weight Loss After Myocardial Infarction in Rats as a Marker of Early Heart Failure Development
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DOI：
10.1016/j.arcmed.2011.06.002
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发表时间：
2011-05-01
影响因子：
7.7
通讯作者：
Stefanon, Ivanita
中科院分区：
文献类型：
--
作者：
Faria, Thais de Oliveira;Baldo, Marcelo Perim;Stefanon, Ivanita
背景和目标。我们评估了大鼠急性心肌梗塞 (MI) 后不久体重 (BW) 下降作为急性心力衰竭 (HF) 标志的用途。方法。对雌性 Wistar 大鼠（200-240 g）进行假手术或冠状动脉闭塞。在各个笼子中，测量每日体重以及食物和水的摄入量。 7天后，通过左心室导管插入术评估心脏功能。心力衰竭的定义是左心室舒张末压大于 95% 置信区间的上限。然后将 MI 组分为发生 HF 的组（n = 27；MI-HF）和未发生 HF 的组（n = 47；MI）。 结果。与其他组相比，MI-HF 组的体重损失增加（假手术：4.2 +/- 0.6% MI：0.4 +/- 0.8%，MI-HF：-4.9 +/- 1.2%；p < 0.05），水和食物摄入量减少。 HF 动物显示出更大的肺重量（假手术：1.460 +/- 0.076 g，MI：1.748 +/- 0.086 g，MI-HF：2.033 +/- 0.13 g；p < 0.05）。各组之间的梗塞面积显着不同（MI：35.9 +/- 0.9%，MI-HF：39.7 +/- 1.3%；p < 0.05）。 ROC 曲线显示，7 天内的体重损失对于识别急性心力衰竭具有 100% 的敏感性和 72.3% 的特异性。此外，排除梗塞面积对这些结果的影响，具有相同梗塞面积的动物样本显示出与整个样本相似的形态测量和血流动力学模式。多元线性回归分析证实，BW 损失是一个独立于梗塞面积的 HF 标志。结论。 BW 是一种简单可靠的无创方法，可在大鼠 MI 后早期检测 HF。 (C) 2011 年IMSS。由爱思唯尔公司出版
Background and Aims. We evaluated the use of body weight (BW) loss soon after acute myocardial infarction (MI) in rats as a marker of acute heart failure (HF).Methods. Female Wistar rats (200-240 g) were submitted either to sham operation or to coronary artery occlusion. In individual cages, daily BW and food and water intake were measured. Seven days later, cardiac function was evaluated by left ventricular catheterization. HF was defined by a left ventricular end-diastolic pressure greater than the upper limit of the 95% confidence interval. MI group was then divided into those that developed HF (n = 27; MI-HF) and those that did not (n = 47; MI).Results. The MI-HF group experienced increased BW loss (sham: 4.2 +/- 0.6% MI: 0.4 +/- 0.8%, MI-HF: -4.9 +/- 1.2%; p < 0.05) and reduced water and food intake compared with other groups. HF animals showed greater lung weight (sham: 1.460 +/- 0.076 g, MI: 1.748 +/- 0.086 g, MI-HF: 2.033 +/- 0.13 g; p < 0.05). Infarct area was significantly different between the groups (MI: 35.9 +/- 0.9%, MI-HF: 39.7 +/- 1.3%; p < 0.05). ROC curve showed that BW loss over 7 days has 100% sensitivity and 72.3% specificity for identifying acute HF. Moreover, excluding the effect of infarct area on these results, a sample of animals with the same infarct area displayed similar morphometric and hemodynamic patterns as the entire sample. Multivariate linear regression analysis confirmed that BW loss is a HF marker independent of infarct area.Conclusions. BW is an easy and reliable noninvasive method to detect HF early after MI in rats. (C) 2011 IMSS. Published by Elsevier Inc.