Onchocerca volvulus -: Exposed persons fail to produce interferon-γ in response to O-volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density
Onchocerca volvulus -: Exposed persons fail to produce interferon-γ in response to O-volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density
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DOI：
10.1086/339820
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发表时间：
2002-04-15
影响因子：
6.4
通讯作者：
Horstmann, RD
中科院分区：
文献类型：
--
作者：
Brattig, NW;Lepping, B;Horstmann, RD
本文研究了551名接触盘尾丝虫的人对盘尾丝虫抗原（OvAg）的细胞免疫应答。在西非加纳的一个高流行地区发生了肠扭转传播。全血分析表明，响应于可溶性O。结果显示，在微丝蚴阴性者血培养物中，肠扭转提取物、细胞增殖以及培养物中IL-5和IL-13的浓度均较高，并随供者微丝蚴计数的增加而显著降低。仅发现干扰素（IFN）-γ的背景浓度，并且这些与MF计数无关。在有丝分裂原的反应中，来自所有人的细胞强烈地与所研究的所有3种细胞因子的增殖和分泌反应。这些发现表明，人外周血细胞对OvAg的反应不包括显著的IFN-γ产生; MF阴性与IL-5和IL-13产生相关，但与先前提出的IFN-γ产生无关; IL-5和IL-13产生随着MF密度的增加而降低。
The cellular immune response to Onchocerca volvulus antigen (OvAg) was studied in 551 persons exposed to O. volvulus transmission in a hyperendemic area of Ghana, West Africa. A whole-blood assay showed that, in response to a soluble O. volvulus extract, cell proliferation, as well as interleukin (IL)-5 and IL-13 concentrations in thesupernatants, were high in cultures of blood from microfilaria (mf)-negative persons and significantly decreased with increasing mf counts of the donors. Only background concentrations of interferon (IFN)-gamma were found, and these did not correlate with mf counts. In response to a mitogen, cells from all persons strongly reacted with proliferation and secretion of all 3 cytokines studied. These findings show that the response of human peripheral blood cells to OvAg does not include significant IFN-gamma production; that mf negativity is associated with IL-5 and IL-13 production but not, as previously suggested, with IFN-gamma production; and that IL-5 and IL-13 production decreases with increasing mf density.