PIRA PCR designer for restriction analysis of single nucleotide polymorphisms
PIRA PCR designer for restriction analysis of single nucleotide polymorphisms
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DOI：
10.1093/bioinformatics/17.9.838
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发表时间：
2001-09-01
期刊：
影响因子：
5.8
通讯作者：
Ye, S
中科院分区：
文献类型：
--
作者：
Ke, XY;Collins, A;Ye, S
引物引入限制性内切酶分析（PIRA-PCR）被广泛用于检测单核苷酸多态性（SNP）。为了产生人工限制性片段长度多态性（RFLP），通常在接近感兴趣的突变的引物的3'末端附近引入错配。我们在这份报告中描述了一个基于www的计算机程序，筛选合适的错配，设计引物，列出合适的限制性内切酶和其他相关信息。
Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the 3' end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.