A dynamic population of stromal cells contributes to the follicle stem cell niche in the Drosophila ovary
A dynamic population of stromal cells contributes to the follicle stem cell niche in the Drosophila ovary
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DOI：
10.1242/dev.098558
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发表时间：
2013-11-15
期刊：
影响因子：
4.6
通讯作者：
Nystul, Todd G.
中科院分区：
文献类型：
--
作者：
Sahai-Hernandez, Pankaj;Nystul, Todd G.
上皮干细胞维持在通过提供指定干细胞命运的信号促进自我更新的小生境内。在果蝇卵巢中，上皮卵泡干细胞（FSC）位于组织前端的壁龛中，并支持卵泡上皮细胞的连续生长。在这里，我们证明了一个相邻的动态群体的基质细胞，称为护送细胞，是FSC龛细胞。我们发现，护送细胞产生无翼和刺猬配体的FSC谱系，无翼信号是特定的FSC生态位，而刺猬信号是活跃的FSC和子细胞。此外，我们发现，多个护送细胞同时封装生殖细胞囊肿和接触FSC。因此，FSC通过非专用的小生境细胞群维持在动态小生境中。
Epithelial stem cells are maintained within niches that promote self-renewal by providing signals that specify the stem cell fate. In the Drosophila ovary, epithelial follicle stem cells (FSCs) reside in niches at the anterior tip of the tissue and support continuous growth of the ovarian follicle epithelium. Here, we demonstrate that a neighboring dynamic population of stromal cells, called escort cells, are FSC niche cells. We show that escort cells produce both Wingless and Hedgehog ligands for the FSC lineage, and that Wingless signaling is specific for the FSC niche whereas Hedgehog signaling is active in both FSCs and daughter cells. In addition, we show that multiple escort cells simultaneously encapsulate germ cell cysts and contact FSCs. Thus, FSCs are maintained in a dynamic niche by a non-dedicated population of niche cells.