The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants
The readthrough region of Potato mop-top virus (PMTV) coat protein encoding RNA, the second largest RNA of PMTV genome, undergoes structural changes in naturally infected and experimentally inoculated plants
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DOI：
10.1007/s007050170156
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Valkonen, JPT
中科院分区：
文献类型：
--
作者：
Sandgren, M;Savenkov, EI;Valkonen, JPT
马铃薯拖把病毒 (PMTV)（马铃薯病毒属）的分子数据目前主要基于对两种苏格兰分离株 PMTV-S 和 PMTV-T 的分析。在这里，我们报告了从瑞典现场获得的 PMTV 分离株的“编码 RNA 的外壳蛋白 (CP)”的完整序列。我们的数据显示，这种 RNA（3134 nt）是 PMTV 三方基因组中三种 RNA 种类中的第二大，因此，它应该被称为 RNA 2。该命名法与其他马铃薯病毒一致。 RNA 2 的通读结构域 (RT) 的序列也在来自芬兰和丹麦的另外两个 PMTV 现场分离株中进行了测定。所有三个分离株在 RT 3' 端都含有一个新的 109 个核苷酸长的序列，这在 PMTV-S 和 PMTV-T 中尚未发现。因此，我们的数据表明先前描述的分离株 PMTV-T 和 PMTV-S 的 RNA 2 序列可能代表缺失衍生物。 RT 的 C 近端部分在分离株之间包含许多氨基酸 (aa) 差异，而 RT 的 N 近端部分仅存在少量氨基酸差异。 RNA 2 的缺失变体是从田间感染的马铃薯块茎和机械接种的测试植物中的北欧分离株中产生的。所有删除均在短区域 (18 nt) 内开始，并从 RT 区域 3' 端移除 558-940 nt。这项研究首次描述了PMTV“CP编码RNA”（RNA2）的全长序列，并揭示了PMTV现场分离株中相当大的aa变异性和RT缺失变异的发生。
Molecular data on Potato mop-top virus (PMTV), genus Pomovirus, is currently mostly based on analysis of two Scottish isolates, PMTV-S and PMTV-T. Here we report the complete sequence of "the coat protein (CP) encoding RNA" of an isolate of PMTV obtained from the field in Sweden. Our data show that this RNA (3134 nt) is the second largest of the three RNA species in the tripartite PMTV genome, and it should, therefore, be referred to as RNA 2. This nomenclature is consistent with other pomoviruses. The sequence of the readthrough domain (RT) of RNA 2 was determined also in two additional field isolates of PMTV from Finland and Denmark. All three isolates contained a novel, 109 nucleotides long sequence at the 3'-end of the RT, which has not been found in PMTV-S and PMTV-T. Hence, our data suggest that the RNA 2 sequences previously described for the isolates PMTV-T and PMTV-S may represent deletion derivatives. The C-proximal half of RT contained many amino acid (aa) differences among the isolates, in contrast to only few aa differences in the N-proximal part of RT. Deletion variants of RNA 2 were generated from the Nordic isolates in potato tubers infected in the field, and in the mechanically inoculated test plants. All deletions started within a short region (18 nt) and removed 558-940 nt from the 3'-end of RT region. This study for the first time describes the full-length sequence of the "CP-encoding RNA" (RNA2) of PMTV, and reveals considerable aa variability and occurrence of deletion variants of RT in the field isolates of PMTV.