Novel and classical human astroviruses in stool and cerebrospinal fluid: comprehensive screening in a tertiary care hospital, Switzerland
Novel and classical human astroviruses in stool and cerebrospinal fluid: comprehensive screening in a tertiary care hospital, Switzerland
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DOI：
10.1038/emi.2017.71
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发表时间：
2017-09-20
影响因子：
13.2
通讯作者：
Kaiser, Laurent
中科院分区：
文献类型：
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作者：
Cordey, Samuel;Vu, Diem-Lan;Kaiser, Laurent
经典人类星状病毒(HAstV)是非细菌性急性胃肠炎的第三大常见原因。由于缺乏常规的分子检测，新的HAstV被低估了，它们对临床疾病的贡献仍不清楚。为了更好地了解它们的患病率和易感患者的特征，我们对一家三级护理医院临床护理收集的粪便和脑脊液(CSF)样本进行了全面的筛查，使用专门设计的RRT-PCR方法检测新型(MLB1-3和VA1-4)和经典HAstV。在654份粪便标本中，有20份HAstV阳性，新的血清型(3份MLB1，4份MLB2，3份VA2)和经典血清型(n=10)同样普遍。105份脑脊液标本无一例阳性。调查患者资料，我们发现儿童粪便中新型和经典甲型肝炎病毒的感染率(分别为3.4%和3%)高于成人粪便样本(分别为0.5%和0.7%)(P=0.0002)。此外，所有新的和经典的HAstV阳性的儿科受试者
Classical human astroviruses (HAstV) are the third most common cause of non-bacterial acute gastroenteritis. Due to the lack of routine molecular assays, novel HAstV are underdiagnosed and the magnitude of their contribution to clinical disease remains unknown. To better understand their prevalence and the susceptible patient profile, we conducted a comprehensive screening of novel and classical HAstV in stool and cerebrospinal fluid (CSF) samples collected for clinical care in a tertiary care hospital using a specially designed rRT-PCR panel for the detection of novel (MLB1-3 and VA1-4) and classical HAstV. Of the 654 stool samples, 20 were positive for HAstV, and the novel (n= 10; 3 MLB1, 4 MLB2; 3 VA2) and classical (n = 10) serotypes were equally prevalent. None of the 105 CSF samples were positive. Investigating the patient profile, we found a higher prevalence (P = 0.0002) of both novel and classical HAstV in pediatric stool samples (3.4% and 3%, respectively) compared with adult stool samples (0.5% and 0.7%, respectively). Furthermore, all novel and classical HAstV-positive pediatric subjects were