The Effect of Dexamethasone on the Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells Treated with Fluvastatin
The Effect of Dexamethasone on the Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells Treated with Fluvastatin
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地塞米松对氟伐他汀处理的人间充质干细胞增殖和成骨细胞分化的影响
DOI：
10.11223/jarde.14.151
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kasahara M.
中科院分区：
文献类型：
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作者：
Tanabe K;Miura T;Yang L;Tsukagoshi E;Yoshinari M;Kasahara M.
他汀类药物被称为 HMG-CoA 还原酶抑制剂，据报道具有成骨作用。然而，地塞米松对他汀类药物诱导的成骨作用的作用机制仍不清楚。本研究探讨了地塞米松 (Dex) 对氟伐他汀 (Flu) 处理的人间充质干细胞 (hMSC) 的细胞增殖和成骨细胞分化的影响。到第 7 天时，添加 10 nM Dex 会增加经 0.5 µM Flu 处理的 hMSC 的生长。此外，在 1.0 µM Flu 存在的情况下，到第 7 天，所有测试浓度的 Dex 都会增加 hMSC 的生长。碱性磷酸酶 (ALP) 活性测定显示，添加 Dex 会导致用 0.5 µM Flu 处理的 hMSC 的 ALP 活性增加，且与浓度无关，而在高 Flu 浓度（包括 1.0 µM 和 2.0 µM）存在的情况下，Dex 不会增加 ALP 水平。这些结果表明，在 0.5 µM Flu 存在的情况下，添加 10 nM Dex 可能是 hMSC 的细胞增殖和成骨细胞分化所必需的。
Statins, known as HMG-CoA reductase inhibitors, were reported to have osteogenic effects. However, the mechanism of the effect of dexamethasone on statin-induced osteogenesis remains elusive. This study investigated the effects of dexamethasone (Dex) on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells (hMSCs) treated with fluvastatin (Flu). The addition of 10 nM Dex increased, by day 7, the growth of hMSCs treated with 0.5 µM Flu. Moreover, Dex of all tested concentrations increased the growth of hMSCs in the presence of 1.0 µM Flu by day 7. An alkaline phosphatase (ALP) activity assay showed the addition of Dex resulted in increased ALP activity of the hMSCs treated with 0.5 µM Flu in a concentration-independent manner, while Dex did not increase ALP levels in the presence of high Flu concentrations including 1.0 µM and 2.0 µM. These results suggest that the addition of 10 nM Dex may be necessary for cell proliferation and osteoblastic differentiation of hMSCs in the presence of 0.5 µM Flu.