Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress.
Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress.
复制标题
维生素C和碳酸氢钠增强H9C2细胞的抗氧化能力并诱导HSP缓解热应激。
DOI：
10.1007/s12192-018-0885-2
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Bao Endong
中科院分区：
文献类型：
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作者：
Yin Bin;Tang Shu;Sun Jiarui;Zhang Xiaohui;Xu Jiao;Di Liangjiao;Li Zhihong;Hu Yurong;Bao Endong
全球变暖加剧了热应激，影响人类和动物健康，导致活性氧（ROS）和抗氧化系统、酸碱化学、电解质和呼吸性碱中毒失衡导致心脏损伤。维生素C清除多余的ROS，碳酸氢钠维持酸碱和电解质平衡，缓解呼吸性碱中毒。在此，我们探讨了单独使用维生素 C 以及与等摩尔碳酸氢钠 (维生素 C-Na) 组合刺激内源性抗氧化剂和热休克蛋白 (HSP) 以缓解 H9C2 细胞热应激的能力。对照组、维生素 C（20 μg/ml 维生素 C，16 小时）和维生素 C-Na（20 μg/ml 维生素 C-Na，16 小时）组热应激 1、3 或 5 小时。治疗组的颗粒和空泡变性、核固缩以及细胞核和线粒体的损伤明显减少，细胞凋亡、乳酸脱氢酶活性、ROS和丙二醛水平也明显减少，而超氧化物歧化酶活性增加。此外，CRYAB、Hsp27、Hsp60 和 Hsp70 mRNA 水平在 3 小时时上调 (p< 0.01)，CRYAB 的蛋白质水平在 0 小时 (p< 0.05) 和 1 小时 (p< 0.01) 时增加，Hsp70 的蛋白质水平在 3 小时和 5 小时时增加 (p< 0.01)。因此，用维生素C或维生素C-Na预处理可能通过增强抗氧化能力和上调CRYAB和Hsp70来保护H9C2细胞免受热损伤。
Heat stress is exacerbated by global warming and affects human and animal health, leading to heart damage caused by imbalances in reactive oxygen species (ROS) and the antioxidant system, acid-base chemistry, electrolytes and respiratory alkalosis. Vitamin C scavenges excess ROS, and sodium bicarbonate maintains acid-base and electrolyte balance, and alleviates respiratory alkalosis. Herein, we explored the ability of vitamin C alone and in combination with equimolar sodium bicarbonate (Vitamin C-Na) to stimulate endogenous antioxidants and heat shock proteins (HSPs) to relieve heat stress in H9C2 cells. Control, vitamin C (20 μg/ml vitamin C for 16 h) and vitamin C-Na (20 μg/ml vitamin C-Na for 16 h) groups were heat-stressed for 1, 3 or 5 h. Granular and vacuolar degeneration, karyopyknosis and damage to nuclei and mitochondria were clearly reduced in treatment groups, as were apoptosis, lactate dehydrogenase activity and ROS and malondialdehyde levels, while superoxide dismutase activity was increased. Additionally, CRYAB, Hsp27, Hsp60 and Hsp70 mRNA levels were upregulated at 3 h (p< 0.01), and protein levels were increased for CRYAB at 0 h (p< 0.05) and 1 h (p< 0.01), and for Hsp70 at 3 and 5 h (p< 0.01). Thus, pre-treatment with vitamin C or vitamin C-Na might protect H9C2 cells against heat damage by enhancing the antioxidant ability and upregulating CRYAB and Hsp70.