REGULATED EXPRESSION OF THE BETA-GLOBIN GENE LOCUS IN SYNTHETIC NUCLEI
REGULATED EXPRESSION OF THE BETA-GLOBIN GENE LOCUS IN SYNTHETIC NUCLEI
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DOI：
10.1101/gad.8.20.2453
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发表时间：
1994-10-15
影响因子：
10.5
通讯作者：
EMERSON, BM
中科院分区：
文献类型：
--
作者：
BARTON, MC;EMERSON, BM
复杂染色体位点内的基因表达调控需要多个核过程。我们分析了克隆的小鸡 β-珠蛋白基因家族在组装成使用非洲爪蟾卵提取物制成的合成细胞核时的转录特性。红细胞蛋白环境中的组装正确地概括了组织特异性染色质结构和染色体位点内的远程启动子-增强子相互作用，从而导致 β-珠蛋白基因激活。在阶段性红细胞蛋白存在的情况下，通过重塑启动子区域内的染色质结构并建立远端启动子-增强子通讯，核小体抑制的β-珠蛋白模板可以通过双链DNA复制被转录激活。基因的编程转录状态（由其染色质结构和长程启动子增强子相互作用编码）对于核解缩和 DNA 复制是稳定的，除非在特定 DNA 结合蛋白存在的情况下发生主动重塑。
Regulated gene expression within a complex chromosomal locus requires multiple nuclear processes. We have analyzed the transcriptional properties of the cloned chick beta-globin gene family when assembled into synthetic nuclei made by use of Xenopus egg extracts. Assembly in an erythroid protein environment correctly recapitulates tissue-specific chromatin structure and long-range promoter-enhancer interaction within the chromosomal locus, resulting in beta-globin gene activation. Nucleosome-repressed beta-globin templates can be transcriptionally activated by double-stranded DNA replication in the presence of staged erythroid proteins by remodeling of the chromatin structure within the promoter region and establishment of distal promoter-enhancer communication. The programmed transcriptional state of a gene, as encoded by its chromatin structure and long-range promoter-enhancer interactions, is stable to nuclear decondensation and DNA replication unless active remodeling occurs in the presence of specific DNA-binding proteins.