Assessing the risk of resistance in Pseudoperonospora cubensis to the fungicide flumorph in vitro.
Assessing the risk of resistance in Pseudoperonospora cubensis to the fungicide flumorph in vitro.
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DOI：
10.1002/ps.1515
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发表时间：
2008-03
影响因子：
4.1
通讯作者：
Shusheng Zhu;Pengfei Liu;Xili Liu;Jianqiang Li;S. Yuan;N. Si
中科院分区：
文献类型：
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作者：
Shusheng Zhu;Pengfei Liu;Xili Liu;Jianqiang Li;S. Yuan;N. Si
背景卵菌类杀菌剂氟吗啉是新近推出的一种羧酸酰胺类杀菌剂。为了评估田间对氟吗啉产生抗药性的风险，作者在获得对这些杀菌剂的抗药性菌株的难易程度、抗药性水平以及它们在实验室的适用性方面，将其与二甲双吗啉和嘧菌酯进行了比较。结果通过适应性筛选和紫外线照射，筛选出对嘧菌酯抗性较高的突变株，其适合度与亲本菌株相当。尝试产生黄瓜假丛孢菌突变株(Burk.和MA Curtis)Rostovsev通过孢子囊适应杀菌剂处理的叶片对氟吗啉和二甲双吗啉的抗性不成功。然而，通过紫外线诱变获得了中等抗性突变体，但它们的抗性水平[最大抗性因子(MRF)<；100]远低于抗嘧菌酯突变株(MRF=733)。除抗性稳定性外，所有突变体的致病力和产孢量均低于野生型菌株和抗菌酯突变体。氟吗啉和二甲双吗啉之间存在交叉抗性，表明它们具有相同的抗性机制。结论氟吗啉的耐药风险可能与二甲双吗啉相似，但低于嘧菌酯，属中度耐药。因此，可以通过适当的产品使用策略对其进行管理。
BACKGROUND The oomycete fungicide flumorph is a recently introduced carboxylic acid amide (CAA) fungicide. In order to evaluate the risk of developing field resistance to flumorph, the authors compared it with dimethomorph and azoxystrobin with respect to the ease of obtaining resistant isolates to these fungicides, the level of resistance and their fitness in the laboratory. RESULTS Mutants with a high level of resistance to azoxystrobin were isolated readily by adaptation and UV irradiation, and their fitness was as good as that of the parent isolates. Attempts to generate mutants of Pseudoperonospora cubensis (Burk. & MA Curtis) Rostovsev resistant to flumorph and dimethomorph by sporangia adaptation on fungicide-treated leaves were unsuccessful. However, moderately resistant mutants were isolated using UV mutagenesis, but their resistance level [maximum resistance factor (MRF) < 100] was much lower than that of the azoxystrobin-resistant mutant (MRF = 733). With the exception of stability of resistance, all mutants showed low pathogenicity and sporulation compared with wild-type isolates and azoxystrobin-resistant mutants. There is cross-resistance between flumorph and dimethomorph, suggesting that they have the same resistance mechanism. CONCLUSION The above results suggest that the resistance risk of flumorph may be similar to that of dimethomorph but lower than that of azoxystrobin and can be classified as moderate. Thus, it can be managed by appropriate product use strategies.