Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes.
Genetic diversity, seasonality and transmission network of human metapneumovirus: identification of a unique sub-lineage of the fusion and attachment genes.
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DOI：
10.1038/srep27730
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发表时间：
2016-06-09
影响因子：
4.6
通讯作者：
Tee KK
中科院分区：
文献类型：
--
作者：
Chow WZ;Chan YF;Oong XY;Ng LJ;Nor'E SS;Ng KT;Chan KG;Hanafi NS;Pang YK;Kamarulzaman A;Tee KK
人偏肺病毒（Human metapneumovirus，HMPV）是一种重要的病毒性呼吸道病原体。目前关于HMPV在生活在热带气候的成人和儿童中的遗传多样性、季节性和传播动力学的知识仍然有限。2012年至2014年期间，在马来西亚吉隆坡出现急性呼吸道感染的个体中，HMPV流行率为2.2%（n = 86/3，935）。在东北季风季节（11 - 4月）观察到季节性高峰，并与较高的相对湿度和雨天数相关（P < 0.05）。融合和附着基因的系统发育分析鉴定了三种已知HMPV亚系A2 b和B1（各30.2%，26/86）和B2（20.9%，18/86）的共循环，2013年观察到基因型从亚系B1转移到A2 b。有趣的是，在18.6%（16/86）的人群中发现了一个以前未被识别的A2亚系。使用基于TN 93成对遗传距离的网络构建自定义脚本，我们确定了多达9个HMPV传播集群，作为多个亚流行病传播。虽然没有观察到明显的大爆发，但在季节性高峰期间传播集群（成对）的频率增加表明传播集群在推动HMPV传播中的潜在作用。我们的研究结果为HMPV的治疗研究、预防策略和疾病暴发监测提供了重要信息。
Human metapneumovirus (HMPV) is an important viral respiratory pathogen worldwide. Current knowledge regarding the genetic diversity, seasonality and transmission dynamics of HMPV among adults and children living in tropical climate remains limited. HMPV prevailed at 2.2% (n = 86/3,935) among individuals presented with acute respiratory tract infections in Kuala Lumpur, Malaysia between 2012 and 2014. Seasonal peaks were observed during the northeast monsoon season (November–April) and correlated with higher relative humidity and number of rainy days (P < 0.05). Phylogenetic analysis of the fusion and attachment genes identified the co-circulation of three known HMPV sub-lineages, A2b and B1 (30.2% each, 26/86) and B2 (20.9%, 18/86), with genotype shift from sub-lineage B1 to A2b observed in 2013. Interestingly, a previously unrecognized sub-lineage of A2 was identified in 18.6% (16/86) of the population. Using a custom script for network construction based on the TN93 pairwise genetic distance, we identified up to nine HMPV transmission clusters circulating as multiple sub-epidemics. Although no apparent major outbreak was observed, the increased frequency of transmission clusters (dyads) during seasonal peaks suggests the potential roles of transmission clusters in driving the spread of HMPV. Our findings provide essential information for therapeutic research, prevention strategies, and disease outbreak monitoring of HMPV.