BASIC FIBROBLAST GROWTH-FACTOR INDUCES RETINAL REGENERATION INVIVO
BASIC FIBROBLAST GROWTH-FACTOR INDUCES RETINAL REGENERATION INVIVO
复制标题
DOI：
10.1016/0012-1606(89)90089-4
复制
发表时间：
1989-07-01
影响因子：
2.7
通讯作者：
HOLLENBERG, MJ
中科院分区：
文献类型：
--
作者：
PARK, CM;HOLLENBERG, MJ
在本研究中，我们研究了碱性成纤维细胞生长因子对22-24期鸡胚视网膜再生的影响。在OVO中，手术切除神经视网膜，保留视网膜色素上皮(RPE)完整，然后将含有碱性成纤维细胞生长因子的缓释塑料植入物植入眼睛。7天后的光镜检查显示，bFGF以剂量依赖的方式诱导视网膜再生。这些眼睛中没有RPE，再生的神经视网膜极性颠倒，这与这个过程是通过RPE的转分化发生的假设是一致的。这是第一次有已知的分子被证明可以在体内诱导视网膜再生。
In the present study, we have investigated the effect of basic fibroblast growth factor (bFGF) on retinal regeneration in the stage 22-24 chick embryo. The neural retina was surgically removed in ovo leaving the retinal pigment epithelium (RPE) intact and then slow-release, plastic implants containing bFGF were inserted into the eye. Light microscopic examination of eyes 7 days later revealed that bFGF induced retinal regeneration in a dose-dependent manner. The absence of the RPE in these eyes and the reversed polarity of the regenerated neural retina is consistent with the hypothesis that this process occurs by transdifferentiation of the RPE. This represents the first time that a known molecule has been shown to induce retinal regeneration in vivo.