Staging of initial pepsinogen and chitinase expression and complete gastric gland development within the larval stomach of Japanese flounder, spotted halibut, seven-band grouper and greater amberjack
Staging of initial pepsinogen and chitinase expression and complete gastric gland development within the larval stomach of Japanese flounder, spotted halibut, seven-band grouper and greater amberjack
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日本比目鱼、斑点大比目鱼、七带石斑鱼和大鰤幼鱼胃内初始胃蛋白酶原和几丁质酶表达以及完整胃腺发育的分期
DOI：
10.1016/j.aquaculture.2011.02.002
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发表时间：
2011
期刊：
影响因子：
4.5
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Xiaoming Wu/Youhei Washio;et al
已知海洋水产养殖鱼类的胃腺在幼虫发育期间开始分泌胃蛋白酶原（Pep），远远在第一次进食之后。虽然胃腺发育的表征被认为是有用的管理幼虫喂养，由胃腺的初始PEP合成的时间只有少数物种的报告。因此，本研究采用原位杂交（ISH）的特点发病的胃酶PEP和几丁质酶（Chi）的基因编码的表达，以及在日本比目鱼（Paralichthys olivaceus），斑点大比目鱼（Verasper variegatus），七带石斑鱼（Epinephalus septemfasciatus）和大琥珀鱼（Seriola dumerili）的幼虫胃胃腺的完整发展。Chi和Pep的表达均开始于仔鱼胃的贲门区，分别为25 d、36 d、38 d和13 d。这些物种的胃腺在Pep表达开始后约5- 12天在胃中完全发育。胃腺的发育与变态的外部表现相一致，如日本比目鱼和斑比目鱼的成年着色的发育，而七带石斑鱼和大琥珀鱼的Perciformes在成年着色之前完成。
The gastric glands of marine aquaculture fish species are known to start secretion of pepsinogens (Pep) during larval development, far after first feeding. Although characterization of gastric gland development is considered useful for managing larval feeding, the timing of initial Pep synthesis by the gastric gland has only been reported for a few species. Consequently, this study employed in situ hybridization (ISH) to characterize the onset of expression of genes encoding the gastric enzymes Pep and Chitinase (Chi), as well as the complete development of gastric glands in the larval stomach of Japanese flounder (Paralichthys olivaceus), spotted halibut (Verasper variegatus), seven-band grouper (Epinephelus septemfasciatus) and greater amberjack (Seriola dumerili). The expression of both Chi and Pep was observed to begin in the cardiac region of the larval stomach at 25days post-hatching (dph) in Japanese flounder, 36dph in spotted halibut, 38dph in seven-band grouper and 13dph in greater amberjack. The gastric glands of these species were fully developed in the stomach approximately 5–12days after the initiation of Pep expression. Development of gastric glands was observed to coincide with external manifestations of metamorphosis, such as the development of adult coloration in Japanese flounder and spotted halibut of the Pleuronectiformes, while it finished prior to adult-type coloration in seven-band grouper and greater amberjack of the Perciformes.