Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia
Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia
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DOI：
10.1016/j.neures.2004.08.015
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发表时间：
2004-12-01
影响因子：
2.9
通讯作者：
Yoon, BW
中科院分区：
文献类型：
--
作者：
Chu, K;Kim, M;Yoon, BW
神经干细胞被认为是多种神经系统疾病细胞替代疗法的候选细胞。为了研究人神经干细胞是否能迁移到成年缺血大鼠脑内，我们在局灶性脑缺血后24小时静脉移植了永生化的人神经干细胞样细胞。在梗死区周围发现静脉注射的人神经干细胞样细胞，在损伤区分化为神经元和星形胶质细胞，移植后存活达56天。加入BrdU后，移植后7 ~ 14天注射细胞数量增加。我们的研究结果表明，静脉注射的人神经干细胞样细胞可能在局部缺血急性期融入缺血性脑，并响应内源性有丝分裂信号进行增殖。(C) 2004爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Neural stem cells are considered as a candidate for cell replacement therapy in various neurological diseases. To investigate whether human neural stem cells can migrate into the adult ischemic rat brain, we transplanted immortalized human neural 'tem-like' cells intravenously 24 h after focal cerebral ischemia. The intravenously injected human neural stem-like cells were found around the infarcted area, differentiated into neurons and astrocytes in the lesioned areas, and survive up to 56 days after transplantation. The number of the injected cells increased between 7 and 14 days after transplantation with incorporating BrdU. Our findings show that intravenously injected human neural stem-like cells may incorporate into the ischemic brain, and undergo proliferation responding to the endogenous mitotic signal during the acute period of focal ischemia. (C) 2004 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.