A NIR Dye for Development of Peripheral Nerve Targeted Probes.
A NIR Dye for Development of Peripheral Nerve Targeted Probes.
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DOI：
10.1039/c2md00297c
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发表时间：
2012-06-01
期刊：
影响因子：
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通讯作者：
Berezin MY
中科院分区：
文献类型：
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作者：
Gustafson TP;Yan Y;Newton P;Hunter DA;Achilefu S;Akers WJ;Mackinnon SE;Johnson PJ;Berezin MY
目前的成像方式缺乏快速评估和分类神经损伤的能力，无法预测有利或不利的愈合结果，而这可以通过早期干预来最大程度地减少慢性疼痛和功能丧失的发作。因此，开发一种无创评估周围神经损伤的技术至关重要。虽然开发能够进行此类诊断的神经特异性近红外 (NIR) 分子探针是我们的长期目标，但仍需要进行初步研究来确定用于构建此类探针的 NIR 染料。我们评估了一种新型高亲水性和可功能化的聚次甲基染料及其疏水性更强的类似物吲哚菁绿在大鼠坐骨神经内注射后的特性。两种染料在神经成像关键深度的报告能力、亲水性对染料通过神经组织转运的重要性以及它们对神经环境的毒性（或缺乏毒性）均已得到评估。结果表明，新型近红外染料是一种合适的荧光报告基因，可用于设计神经特异性光学分子探针，用于神经损伤的非侵入性诊断和分类。
Current imaging modalities lack the ability to quickly assess and classify nerve injury for predicting favourable versus unfavourable healing outcomes, which could minimize episodes of chronic pain and loss of function by allowing for early intervention. Thus, the development of a technique to noninvasively assess peripheral nerve damage is of critical importance. While the development of nerve specific near infrared (NIR) molecular probes capable of such diagnostics constitutes our long term goal, initial studies to identify a NIR dye for constructing such a probe are required. We have evaluated the properties of a novel highly hydrophilic and functionalizable polymethine dye, and its more hydrophobic analogue indocyanine green, within the sciatic nerve of rats following intra-nerve injection. The reporting ability of both dyes at critical depths for nerve imaging, the importance of hydrophilicity on dye transport through nervous tissue, and their toxicity – or lack thereof – to the neural environment have been evaluated. The results suggest that the novel NIR dye is an appropriate fluorescent reporter for use in designing nerve-specific optical molecular probes for non-invasive diagnosis and classification of nerve injury.