Mapping of Bacterial Blight Resistance Gene Xa11 on Rice Chromosome 3
Mapping of Bacterial Blight Resistance Gene Xa11 on Rice Chromosome 3
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DOI：
10.6090/jarq.43.221
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发表时间：
2009-07-01
影响因子：
0.4
通讯作者：
Yoshimura, Atsushi
中科院分区：
文献类型：
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作者：
Goto, Takahiro;Matsumoto, Tadayuki;Yoshimura, Atsushi
白叶枯病（BB）抗性基因Xa 11赋予水稻对BB病原体黄单胞菌致病型的日本小种IB、II IIIA和V的抗性。水稻（Xoo）。在这里，我们报告了使用随机扩增多态性DNA（RAPD）、切割扩增多态性序列（CAPS）和简单序列重复（SSR）标记对Xa 11进行的定位。为了检测与Xa 11连锁的DNA标记，我们利用Xa 11近等基因系IR-BB 11（在IR 24的遗传背景下）和感病品种IR 24。鉴定了RAPID片段L19（1200），即与Xa 11连接的Purific。L19（1200）产物的克隆和测序表明，它以登录号AC 097277保存在水稻基因组计划数据库中，并且它存在于3号染色体上。根据L19（1200）及其侧翼基因组序列，设计了CAPS标记KUX 11，并筛选出两个SSR标记RM 347和RM 1350用于Xa 11的定位。为了证实Xa 11和这三个标记之间的推定连锁，我们使用IR 24和IR-BB 11之间的杂交的F群体进行连锁分析。用菌株T7156（小种IB）接种每株F-2植物，并用KUX 11、RM 347和RM 1350进行基因分型。在F中，Xa 11位于3号染色体长臂上RM 347（2.0 cM）和KUX 11（1.0 cM）之间。这些结果为Xa 11的分子标记辅助选择和图位克隆提供了依据。
The bacterial blight (BB) resistance gene Xa11 confers resistance in rice to Japanese races IB, II IIIA, and V of the BB pathogen Xanthomonas oryzae pv. oryzae (Xoo). Here, we report the mapping, of Xa11 by using randomly amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sequence (CAPS), and simple sequence repeat (SSR) markers. To detect DNA markers linked to Xa11, We used an Xa11 near-isogenic line, IR-BB11 (in genetic background of IR24), and the susceptible cultivar IR24. The RAPID fragment L19(1200), Putatively linked to Xa11, was identified. Cloning and sequencing of the L19(1200), product indicated that it was held in the Rice Genome Program database as accession number AC097277, and that it occurs oil chromosome 3. On the basis of the sequences of L19(1200) and the flanking genomic region, we designed CAPS marker KUX11 and selected two SSR markers, RM347 and RM1350, for mapping Xa11. To confirm the Putative linkage among Xa11 and the three markers, we conducted linkage analysis using the F, Population of a cross between IR24 and IR-BB11. Each F-2 plant was inoculated with strain T7156 (race IB) and genotyped with KUX11, RM347, and RM1350. Segregation in the F, located Xa11 between the loci of RM347 (2.0 cM) and KUX11 (1.0 cM) oil the long arm of chromosome 3. These results should be useful for the marker-assisted selection of Xa11 in breeding Programs and for cloning Xa11 by map-based cloning.