Aluminium fluoride associates with the small guanine nucleotide binding proteins
Aluminium fluoride associates with the small guanine nucleotide binding proteins
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DOI：
10.1016/s0014-5793(97)00422-5
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发表时间：
1997-05-26
期刊：
影响因子：
3.5
通讯作者：
Wittinghofer, A
中科院分区：
文献类型：
--
作者：
Ahmadian, MR;Mittal, R;Wittinghofer, A
长期以来，人们都知道AlF 4-可以与异源三聚体G蛋白的GDP结合α亚基结合并激活。最近，小鸟嘌呤核苷酸结合蛋白Ras也被证明在化学计量量的GTP酶激活蛋白（GAP）存在下与AlF 4-缔合，本文提出了通过凝胶过滤分离稳定的Ras·GDP·AlF 4-·GAP三元复合物。此外，我们通过证明Cdc 42的三元复合物形成，Rap和Ran蛋白在它们各自的GAP蛋白存在下。(C)1997年欧洲生物化学学会联合会。
AlF4- has long been known to associate with and activate the GDP-bound alpha subunits of heterotrimeric G-proteins. Recently the small guanine nucleotide binding protein Ras has also been shown to associate with AlF4- in the presence of stoichiometric amounts of its GTPase activating protein (GAP), Here we present the isolation of a stable Ras.GDP.AlF4-.GAP ternary complex by gel filtration, In addition, we generalise the association of AlF4- with the small GTP-binding proteins by demonstrating ternary complex formation for the Cdc42, Rap and Ran proteins in the presence of their respective GAP proteins. (C) 1997 Federation of European Biochemical Societies.