Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction
Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction
复制标题
急性心肌梗死Wistar大鼠缺血心肌差异基因表达谱
DOI：
10.1007/s11434-008-0333-2
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发表时间：
2008
影响因子：
--
通讯作者：
D. Shi
中科院分区：
文献类型：
--
作者：
Chun;H. Yin;Yuerong Jiang;Mei Xue;D. Shi
为了确定患有急性心肌梗死（AMI）的 Wistar 大鼠缺血心肌中的差异基因，我们构建了两个差异基因表达谱。 AMI模型是通过结扎Wistar大鼠左冠状动脉前降支而产生的。术后第8天，从结扎点下的正常心脏组织和缺血心脏组织中提取总RNA。通过使用基因表达的长序列分析（LongSAGE）构建了两个样本的差异基因表达谱。采用实时荧光定量PCR（Q-PCR）确认部分目的基因的表达变化。主要结果如下：从正常和缺血LongSAGE图谱中共筛选出15966个标签，获得正常组织中9646个标签和缺血组织中9563个标签。其中，NCBI BLAST 搜索识别出 7665 个新颖标签。缺血组织中有142个基因与正常组织相比发生显着变化（P<0.05）。这些差异表达基因可能在氧化和磷酸化、ATP合成和糖酵解等途径中发挥重要作用。 LongSAGE 鉴定的部分基因通过 Q-PCR 得到证实。结果表明，AMI在能量代谢相关通路的调节中引起一系列基因表达变化。
To determine the differential genes in ischemic myocardium of Wistar rats with acute myocardial infarction (AMI), we constructed two differential gene expression profiles. AMI model was generated by ligation of the left anterior descending coronary artery in Wistar rats. Total RNA was extracted from the normal and the ischemic heart tissues under the ligation point at the 8th day after the operation. Differential gene expression profiles of the two samples were constructed by using long serial analysis of gene expression (LongSAGE). Real time fluorescence quantitative PCR (Q-PCR) was used to confirm the expression changes of partial target genes. The main results were as follows: a total of 15966 tags were screened from the normal and the ischemic LongSAGE maps, and 9646 tags in the normal tissue and 9563 tags in the ischemic tissue were obtained. Among them, 7665 novel tags were identified by NCBI BLAST search. In the ischemic tissue, 142 genes significantly changed compared to those in the normal tissue (P<0.05). These differentially expressed genes may play important roles in the pathways of oxidation and phosphorylation, ATP synthesis and glycolysis and so on. Partial genes identified by the LongSAGE were confirmed by Q-PCR. The results show that AMI causes a series of gene expression changes in the regulation of the pathways related to energy metabolism.
影响因子：
13.8
作者：
K. Webster
通讯作者：
K. Webster