Adolescent Social Stress Increases Anxiety-like Behavior and Alters Synaptic Transmission, Without Influencing Nicotine Responses, in a Sex-Dependent Manner
Adolescent Social Stress Increases Anxiety-like Behavior and Alters Synaptic Transmission, Without Influencing Nicotine Responses, in a Sex-Dependent Manner
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DOI：
10.1016/j.neuroscience.2018.01.006
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发表时间：
2018-03-01
期刊：
影响因子：
3.3
通讯作者：
Kamens, Helen M.
中科院分区：
文献类型：
--
作者：
Caruso, Michael J.;Crowley, Nicole A.;Kamens, Helen M.
早期生活压力是焦虑和尼古丁使用共病的危险因素。由于对这种共病的潜在因素知之甚少，我们研究了青少年压力对个体动物焦虑样行为和尼古丁反应的影响。将青春期雄性和雌性C57 BL/6 J小鼠从出生后第25-59天（PND）暴露于慢性可变社会应激（CVSS;社会隔离+社会重组的重复循环）或对照条件。分别在高架十字迷宫（PND 61-65）和社交接近-回避测试（实验1：PND 140-144;实验2：95-97）中测量了行为学样行为和社交回避。还检查了急性尼古丁诱导的运动、体温过低、皮质酮反应（实验1：PND 56-59;实验2：PND 65-70）和自愿口服尼古丁消耗（实验1：PND 116-135;实验2：73-92）。最后，我们评估了前额叶皮层（PFC）和神经核（NAC）的突触传递（PND 64-80）;与焦虑和成瘾有关的大脑区域。与对照组相比，暴露于青少年CVSS的小鼠表现出焦虑样行为增加。此外，CVSS改变突触兴奋性PFC和NAC神经元的性别特异性的方式。对于男性，CVSS的PFC和NAC的自发兴奋性突触后电流的幅度和频率降低，分别。在女性中，CVSS降低了NAC中自发抑制性突触后电流的幅度。青少年CVSS不影响社交回避或尼古丁反应，焦虑样行为与个别动物的尼古丁反应不可靠。总而言之，PFC和NAC功能之间的复杂相互作用可能有助于青少年压力诱导的焦虑样行为，而不影响尼古丁反应。(C)2018年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Early-life stress is a risk factor for comorbid anxiety and nicotine use. Because little is known about the factors underlying this comorbidity, we investigated the effects of adolescent stress on anxiety-like behavior and nicotine responses within individual animals. Adolescent male and female C57BL/6J mice were exposed to chronic variable social stress (CVSS; repeated cycles of social isolation + social reorganization) or control conditions from postnatal days (PND) 25-59. Anxiety-like behavior and social avoidance were measured in the elevated plus-maze (PND 61-65) and social approach-avoidance test (Experiment 1: PND 140-144; Experiment 2: 95-97), respectively. Acute nicotine-induced locomotor, hypothermic, corticosterone responses, (Experiment 1: PND 56-59; Experiment 2: PND 65-70) and voluntary oral nicotine consumption (Experiment 1: PND 116-135; Experiment 2: 73-92) were also examined. Finally, we assessed prefrontal cortex (PFC) and nucleus accumbens (NAC) synaptic transmission (PND 64-80); brain regions that are implicated in anxiety and addiction. Mice exposed to adolescent CVSS displayed increased anxiety-like behavior relative to controls. Further, CVSS altered synaptic excitability in PFC and NAC neurons in a sex-specific manner. For males, CVSS decreased the amplitude and frequency of spontaneous excitatory postsynaptic currents in the PFC and NAC, respectively. In females, CVSS decreased the amplitude of spontaneous inhibitory postsynaptic currents in the NAC. Adolescent CVSS did not affect social avoidance or nicotine responses and anxiety-like behavior was not reliably associated with nicotine responses within individual animals. Taken together, complex interactions between PFC and NAC function may contribute to adolescent stress-induced anxiety-like behavior without influencing nicotine responses. (C) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.