A survey of growth in soft agar and cell surface properties as markers for transformation in adult rat liver epithelial-like cell cultures.
A survey of growth in soft agar and cell surface properties as markers for transformation in adult rat liver epithelial-like cell cultures.
复制标题
对软琼脂生长和细胞表面特性作为成年大鼠肝上皮样细胞培养物转化标记的调查。
作者：
R. San;M. F. Laspia;A. Soiefer;C. J. Maslansky;J. Rice;G. Williams
连续上皮样细胞系来自正常成年大鼠肝脏和肝癌进行了评价，其生长在软琼脂和五个属性的细胞膜作为标志物的肿瘤转化。这些属性的相关性进行了裸鼠的致瘤性的线。软琼脂中的生长是一种特异性且敏感的标志物，而2-脱氧-D-葡萄糖吸收的数据是一致的，高吸收是一种特异性但显然不是敏感的标志物。伴刀豆球蛋白A介导的凝集和红细胞吸附，细胞松弛素B存在下的多核化，以及三磷酸腺苷酶的细胞膜活性与其他转化标志物的致瘤性无关。此外，还表明支原体感染不会改变这些特性中的任何一种，但可以通过细胞在裸鼠体内传代来消除感染。
Continuous epithelial-like cell lines derived from normal adult rat liver and hepatocarcinomas were evaluated for their growth in soft agar and five properties of the cell membrane as markers for neoplastic transformation. A correlation of these properties was made to the tumorigenicity of the lines in nude mice. Growth in soft agar was a specific and sensitive marker, whereas the data on uptake of 2-deoxy-D-glucose were consistent, with high uptake being a specific but clearly not a sensitive marker. Agglutination and hemadsorption mediated by concanavalin A, multinucleation in the presence of cytochalasin B, and the cell membrane activity of adenosine triphosphatase did not correlate with tumorigenicity of the other markers for transformation. In addition, it is shown that Mycoplasma infection does not alter any of these properties but that infection can be eliminated by passage of cells through nude mice.