The effect of a recombinant elastin-mimetic coating of an ePTFE prosthesis on acute thrombogenicity in a baboon arteriovenous shunt
The effect of a recombinant elastin-mimetic coating of an ePTFE prosthesis on acute thrombogenicity in a baboon arteriovenous shunt
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DOI：
10.1016/j.biomaterials.2006.09.048
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Chaikof, Elliot L.
中科院分区：
文献类型：
--
作者：
Jordan, Sumanas W.;Haller, Carolyn A.;Chaikof, Elliot L.
使用具有逆转变温度（类似于20 ° C）的重组弹性蛋白模拟物三嵌段蛋白聚合物将小直径（4 mm i.d.）膨体聚四氟乙烯（ePTFE）血管移植物。扫描电子显微镜证实，移植物的初始弹性蛋白浸渍，随后用弹性蛋白膜进一步多层涂覆，填充在ePTFE移植物的管腔表面的原纤维和节点结构中，并且宏观上是光滑的。弹性蛋白质聚合物浸渍将管腔表面的前进接触角减小至43度，这与浇铸弹性蛋白膜的47度的前进接触角相当。衰减全反射红外光谱和考马斯亮蓝染色显示，在500 s（-1）和37 ℃下剪切24 h后，蛋白质表面膜几乎没有可辨别的变化。使用狒狒体外股动静脉分流模型证明了通过最小纤维蛋白和血小板沉积确定的极佳短期血液接触特性。本研究的结果证明了弹性蛋白模拟三嵌段蛋白聚合物作为非血栓形成涂层或作为组织工程复合材料的组分的适用性。(c)2006爱思唯尔有限公司保留所有权利。
A recombinant elastin-mimetic triblock protein polymer with an inverse transition temperature (similar to 20 degrees C) was used to impregnate small-diameter (4mm i.d.) expanded polytetrafluoroethylene (ePTFE) vascular grafts. Scanning electron microscopy confirmed that initial elastin impregnation of the graft followed by further multilayer coating with elastin films filled in the fibril and node structure of the luminal surface of the ePTFE graft and was macroscopically smooth. Elastin protein polymer impregnation reduced the advancing contact angle of the luminal surface to 43 degrees, which was comparable to the advancing contact angle of 47 degrees for a cast elastin film. Attenuated total reflection infrared spectroscopy and Coomassie blue staining revealed little discernable change in the protein surface film after 24h of shear at 500s(-1) and 37 degrees C. Excellent short-term blood-contacting properties as determined by minimal fibrin and platelet deposition were demonstrated using a baboon extracorporeal femoral arteriovenous shunt model. The results of this study demonstrate the applicability of an elastin-mimetic triblock protein polymer as a non-thrombogenic coating or as a component of a tissue-engineered composite. (c) 2006 Elsevier Ltd. All rights reserved.