Increased levels of circulating SDF-1α and CD34+ CXCR4+ cells in patients with moyamoya disease
Increased levels of circulating SDF-1α and CD34+ CXCR4+ cells in patients with moyamoya disease
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DOI：
10.1111/j.1468-1331.2011.03393.x
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发表时间：
2011-11-01
影响因子：
5.1
通讯作者：
Xu, G.
中科院分区：
文献类型：
--
作者：
Ni, G.;Liu, W.;Xu, G.
背景与目的：最近的研究报道，外周血CD34（+）细胞和内皮祖细胞参与烟雾病（MMD）患者的血管生成，基质细胞衍生因子-1 α (SDF-1 α)通过与相应的CXCR4受体相互作用调节血管生成。我们在本研究中调查了SDF-1 α /CXCR4轴是否参与烟雾病患者的新生血管形成。方法：选取18例烟雾病患者和12例健康人。通过脑血管造影检测的烟雾病患者从南京市卒中登记项目中检索。外周全血细胞用抗cd34和抗cxcr4 （CD184）双染色。流式细胞术分析CD34(+) CXCR4（+）细胞数量。采用酶联免疫吸附法测定血浆中SDF-1a的浓度。结果：在连续的烟雾病患者中，CD34(+) CXCR4（+）细胞数量大于健康个体(54.1 +/- 28.6/ μ l外周血（PB） vs . 24.6 +/- 25.6/ μ l PB；P = 0.015)。MMD患者血浆SDF-1 α水平显著高于健康对照组（1676.76 +/- 198.65 pg/ml vs 1508.26 +/- 137.27 pg/ml; P = 0.016）。MMD患者CD34(+) CXCR4（+）细胞数量与血浆SDF-1 α水平无显著相关性（r = 0.185; P = 0.46）。结论：本研究提示烟雾病患者循环中SDF-1 α和CD34(+) CXCR4（+）细胞水平升高，可能在烟雾病患者血管发生中发挥重要作用。
Background and Purpose: Recent studies reported that peripheral blood CD34(+) cells and endothelial progenitor cells contributed to angiogenesis in patients with moyamoya disease (MMD), and stromal cell-derived factor-1 alpha (SDF-1 alpha) modulated angiogenesis by interacting with corresponding CXCR4 receptors. We investigated in this study whether SDF-1 alpha/CXCR4 axis involved in neovascularization in patients with MMD.Methods: Eighteen patients with MMD and twelve healthy individuals were enrolled. Patients with MMD detected by cerebral angiography were retrieved from the Nanjing Stroke Registry Program. Peripheral whole blood cells were double stained with anti-CD34 and anti-CXCR4 (CD184). Numbers of CD34(+) CXCR4(+) cells were analyzed by flow cytometry. Plasma concentration of SDF-1a was determined by enzyme-linked immunosorbent assay.Results: In consecutive patients with MMD, the number of CD34(+) CXCR4(+) cells was greater than healthy individuals (54.1 +/- 28.6/mu l peripheral blood (PB) versus 24.6 +/- 25.6/mu l PB; P = 0.015). Plasma SDF-1 alpha level was significantly higher in patients with MMD compared with healthy controls (1676.76 +/- 198.65 pg/ml versus 1508.26 +/- 137.27 pg/ml; P = 0.016). No significant correlation between number of CD34(+) CXCR4(+) cells and plasma SDF-1 alpha level in the patients with MMD was observed (r = 0.185; P = 0.46).Conclusion: This study indicated that increased levels of circulating SDF-1 alpha and CD34(+) CXCR4(+) cells in patients with MMD, which may play an important role in the vasculogenesis in MMD.