Field evaluation of Crystal VC® Rapid Dipstick test for cholera during a cholera outbreak in Guinea-Bissau
Field evaluation of Crystal VC® Rapid Dipstick test for cholera during a cholera outbreak in Guinea-Bissau
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DOI：
10.1111/j.1365-3156.2009.02335.x
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发表时间：
2009-09-01
影响因子：
3.3
通讯作者：
Mintz, Eric D.
中科院分区：
文献类型：
--
作者：
Harris, Julie R.;Cavallaro, Elizabeth C.;Mintz, Eric D.
为了评估新的市售Crystal VC（R）快速试纸条（VC）检测（Span Diagnostics，India）用于霍乱弧菌O 1和O 139的性能特征和易用性。方法收集2008年几内亚比绍霍乱流行期间医院霍乱病房患者的全粪便。现场对粪便样本进行VC检测;随后将样本储存在Cary-Blair转运培养基中，并送往疾病控制和预防中心，通过培养和聚合酶链反应（PCR）进行诊断检测。此外，四个当地的实验室技术人员谁是不熟悉的测试提供了粪便样本，VC测试试剂盒，和简单的书面说明，并要求进行测试和解释result.Results共收集和测试101粪便标本。与PCR相比，敏感性为97%，特异性为71-76%。在比绍的实验室技术人员进行测试和解释结果正确使用简单的书面instructions.CONCLUSIONS VC测试可能是有用的霍乱爆发的情况下，实验室能力是有限的诊断。
OBJECTIVES To evaluate performance characteristics and ease of use of the new commercially available Crystal VC (R) Rapid Dipstick (VC) test (Span Diagnostics, India) for Vibrio cholerae O1 and O139.METHODS Whole stool was collected from patients presenting to a hospital cholera ward during a 2008 epidemic in Guinea-Bissau. The VC test on stool samples was conducted on-site; samples were subsequently stored in Cary-Blair transport media and sent to the Centers for Disease Control and Prevention for diagnostic testing by culture and polymerase chain reaction (PCR). In addition, four local laboratory technicians who were unfamiliar with the test were provided with stool samples, the VC test kit, and simple written instructions and asked to perform the test and interpret results.RESULTS A total of 101 stool specimens were collected and tested. Compared with PCR, the test was 97% sensitive and 71-76% specific. Laboratory technicians in Bissau performed the test and interpreted results correctly using only simple written instructions.CONCLUSIONS The VC test may be useful for cholera diagnosis in outbreak situations where laboratory capacity is limited.