Burkholderia silvatlantica sp nov., a diazotrophic bacterium associated with sugar cane and maize
Burkholderia silvatlantica sp nov., a diazotrophic bacterium associated with sugar cane and maize
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DOI：
10.1099/ijs.0.64362-0
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发表时间：
2006-08-01
影响因子：
2.8
通讯作者：
Caballero-Mellado, J.
中科院分区：
文献类型：
--
作者：
Perin, L.;Martinez-Aguilar, L.;Caballero-Mellado, J.
在之前的一项研究中，从巴西里约热内卢种植的玉米根际和表面灭菌的甘蔗叶子中回收了固氮分离株。根据 16S rRNA 基因序列相似性，这些分离株被鉴定属于伯克霍尔德氏菌属，全细胞蛋白质谱表明它们彼此密切相关。在本研究中，从巴西不同地理区域种植的不同甘蔗品种的根中回收了新的分离株。使用多相分类学标准对 21 个固氮分离株进行了分析，包括 DNA-DNA 相关性、16S rRNA 基因序列相似性、脂肪酸谱、全细胞蛋白质模式和多位点酶电泳谱，以及形态、生理和生化特征。分析证实，这些分离株属于伯克霍尔德氏菌属中的一个新种，其名称为Burkholderia silvatlantica sp。十一月被提议。模式菌株 SRMrh-20(T)（=LMG 23149(T)=ATCC BAA-1244(T)）是从玉米 var. 的根际分离出来的。 Avantis A2345 种植于里约热内卢的Seropedica。
In a previous study, nitrogen-fixing isolates were recovered from the rhizosphere of maize and from surface-sterilized leaves of sugar cane cultivated in Rio de Janeiro, Brazil. On the basis of 16S rRNA gene sequence similarities, these isolates were identified as belonging to the genus Burkholderia, and whole-cell-protein profiles demonstrated that they are closely related to each other. In the present study, novel isolates were recovered from the roots of different sugar-cane varieties cultivated in diverse geographical regions of Brazil. Twenty-one nitrogen-fixing isolates were analysed using polyphasic taxonomy criteria, including DNA-DNA relatedness, 16S rRNA gene sequence similarities, fatty acid profiles, whole-cell-protein patterns and multilocus enzyme electrophoresis profiles, as well as morphological, physiological and biochemical characterization. The analysis confirmed that these isolates belong to a novel species within the genus Burkholderia, for which the name Burkholderia silvatlantica sp. nov. is proposed. The type strain, SRMrh-20(T) (=LMG 23149(T) =ATCC BAA-1244(T)), was isolated from the rhizosphere of maize var. Avantis A2345 cultivated in Seropedica, Rio de Janeiro.